Juliana Jasper1, Sérgio Roithmann2, Renata Stifelman Camilotti3, Fernanda Gonçalves Salum4, Karen Cherubini5, Maria Antonia Zancanaro de Figueiredo5. 1. MSc Student, Oral Medicine Department, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil. Electronic address: jujasper@hotmail.com. 2. Head of the Oncology Service of Associação Hospitalar Moinhos de Vento, Professor of Medicine of Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, RS, Brazil. 3. MSc Student, Oral and Maxillofacial Surgery Department, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil. 4. Senior Lecturer of Oral Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil. 5. Professor of Oral Medicine, Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.
Abstract
OBJECTIVE: To investigate the effect of granulocyte colony-stimulating factor (G-CSF) on oral mucositis (OM) and on the healing of traumatic ulcers produced in the tongue of rats undergoing radiotherapy (RT). STUDY DESIGN: Twenty-seven Wistar rats were assigned to four groups: (1) RT + traumatic ulcer + filgrastim (G-CSF analog; n = 7); (2) RT + traumatic ulcer + saline (n = 7); (3) no RT + traumatic ulcer + filgrastim (n = 7); and (4) no RT + traumatic ulcer (n = 6). The radiation dose was 30 Gy, and medication was filgrastim (10 μg/kg) for 7 days. RESULTS: Clinically, groups differed in the presence (Fisher's exact test: P = .008) and size of traumatic ulcers after irradiation (Kruskal-Wallis test: P = .032) and in the severity of OM (Fisher's exact test: P = .005 between the irradiated groups). Histologically, there was an increased inflammatory response in the nonirradiated groups (Fisher's exact test: P = .001). CONCLUSIONS: Filgrastim reduced manifestations and the severity of trauma-induced ulcers and radiation-induced OM. Significant differences were not observed histologically between the study drug and respective control groups.
OBJECTIVE: To investigate the effect of granulocyte colony-stimulating factor (G-CSF) on oral mucositis (OM) and on the healing of traumatic ulcers produced in the tongue of rats undergoing radiotherapy (RT). STUDY DESIGN: Twenty-seven Wistar rats were assigned to four groups: (1) RT + traumatic ulcer + filgrastim (G-CSF analog; n = 7); (2) RT + traumatic ulcer + saline (n = 7); (3) no RT + traumatic ulcer + filgrastim (n = 7); and (4) no RT + traumatic ulcer (n = 6). The radiation dose was 30 Gy, and medication was filgrastim (10 μg/kg) for 7 days. RESULTS: Clinically, groups differed in the presence (Fisher's exact test: P = .008) and size of traumatic ulcers after irradiation (Kruskal-Wallis test: P = .032) and in the severity of OM (Fisher's exact test: P = .005 between the irradiated groups). Histologically, there was an increased inflammatory response in the nonirradiated groups (Fisher's exact test: P = .001). CONCLUSIONS: Filgrastim reduced manifestations and the severity of trauma-induced ulcers and radiation-induced OM. Significant differences were not observed histologically between the study drug and respective control groups.