Luiza Cassiano1, Juliano Pessan2, Livia Comar3, Flavia Levy4, Cristiane Cardoso5, Aline Dionisio6, Michele Manarelli7, Larissa Grizzo8, Ana Carolina Magalhães9, Marília Buzalaf10. 1. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil. Electronic address: luiza.cassiano@usp.br. 2. Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, SP, R. José Bonifácio, 1193, Araçatuba, SP, 16015-050, Brazil. Electronic address: jpessan@foa.unesp.br. 3. School of Dentistry, Univ. Cidade de São Paulo (UNICID), R. Cesário Galeno, 448/475, São Paulo-SP, 03071-000, Brazil. Electronic address: liviacomar@yahoo.com.br. 4. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil. Electronic address: flalevy@usp.br. 5. Institute of Dentistry, Cruzeiro do Sul University, R. Galvão Bueno, 868, São Paulo-SP, 01506-000, Brazil. Electronic address: crisbaldini@usp.br. 6. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil. Electronic address: stars_line@hotmail.com. 7. Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, SP, R. José Bonifácio, 1193, Araçatuba, SP, 16015-050, Brazil. Electronic address: michelemanarelli@gmail.com. 8. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil. Electronic address: larissagrizzo@yahoo.com.br. 9. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil. Electronic address: acm@fob.usp.br. 10. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil. Electronic address: mbuzalaf@fob.usp.br.
Abstract
OBJECTIVES: This study analysed the effect of frequency of intake and amount of fluoride in milk on the remineralisation of artificial enamel and dentine caries lesions ex vivo/in situ. MATERIALS AND METHODS: Pre-demineralised bovine enamel and dentine slabs were randomly allocated into 5 groups and fixed in removable appliances used by subjects for 7days in each phase. Each treatment comprised milk containing 2.5ppm fluoride daily (T1), or every other day (T2), 5.0ppm F daily (T3), or every other day (T4) or no treatment (T5). RESULTS: Enamel alterations were quantified by surface hardness recovery (%SHR) and transversal microradiography (TMR), and in dentine by TMR only. Data were analysed by ANOVA and Tukey's test (p<0.05). For enamel, the highest %SHR was found for T1 and T3 compared to control, without significant differences between them. All groups showed positive values of ΔΔZ - T1 (247.3±198.5); T2 (110.9±303.2); T3 (226.0±299.2); T5 (5.0±288.0), except T4 (-274.5±407.3). For dentine, the only group that presented remineralisation was T2 (350.0±657.5). CONCLUSIONS: Fluoridated milk daily seems to have higher remineralising effect on enamel than its use every other day. Dentine, does not seem to benefit from daily use of fluoridated milk. Copyright Â
RCT Entities:
OBJECTIVES: This study analysed the effect of frequency of intake and amount of fluoride in milk on the remineralisation of artificial enamel and dentine caries lesions ex vivo/in situ. MATERIALS AND METHODS: Pre-demineralised bovine enamel and dentine slabs were randomly allocated into 5 groups and fixed in removable appliances used by subjects for 7days in each phase. Each treatment comprised milk containing 2.5ppm fluoride daily (T1), or every other day (T2), 5.0ppm F daily (T3), or every other day (T4) or no treatment (T5). RESULTS: Enamel alterations were quantified by surface hardness recovery (%SHR) and transversal microradiography (TMR), and in dentine by TMR only. Data were analysed by ANOVA and Tukey's test (p<0.05). For enamel, the highest %SHR was found for T1 and T3 compared to control, without significant differences between them. All groups showed positive values of ΔΔZ - T1 (247.3±198.5); T2 (110.9±303.2); T3 (226.0±299.2); T5 (5.0±288.0), except T4 (-274.5±407.3). For dentine, the only group that presented remineralisation was T2 (350.0±657.5). CONCLUSIONS: Fluoridated milk daily seems to have higher remineralising effect on enamel than its use every other day. Dentine, does not seem to benefit from daily use of fluoridated milk. Copyright Â