Daniel Haselbach1, Wassim Raffoul2, Lorenz Larcher3, Mathias Tremp4, Daniel F Kalbermatten5, Pietro G di Summa6. 1. Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Lausanne (CHUV), Rue du Bugnon 46, 1011 Lausanne, Switzerland. Electronic address: Daniel.Haselbach@chuv.ch. 2. Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Lausanne (CHUV), Rue du Bugnon 46, 1011 Lausanne, Switzerland. Electronic address: Wassim.Raffoul@chuv.ch. 3. Department of Plastic, Reconstructive and Hand Surgery, Brixen Hospital, South Tyrolean Health Care Service (SABES), Dantersrasse 51-I-3942, Brixen, Italy. Electronic address: lorenz.larcher@gmail.com. 4. Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Basel, Spitalstrasse 21, CH-4031 Basel, Switzerland. Electronic address: Mathias.Tremp@usb.ch. 5. Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Basel, Spitalstrasse 21, CH-4031 Basel, Switzerland. Electronic address: Daniel.Kalbermatten@usb.ch. 6. Department of Plastic, Reconstructive and Hand Surgery, University Hospital of Lausanne (CHUV), Rue du Bugnon 46, 1011 Lausanne, Switzerland. Electronic address: Pietro.di-Summa@chuv.ch.
Abstract
INTRODUCTION: Hindlimb autophagy is common after rat sciatic total axotomy and is considered as a sign of neuropathic pain. We applied adult stem cells in a fibrin conduit in a total sciatic axotomy model to improve nerve regeneration, investigating whether a correlation could be detected between stem cells effects on regeneration and limb autophagy. MATERIAL AND METHODS: After sciatic nerve section, a 1-cm sciatic gap was crossed using fibrin conduits. Experimental groups included empty fibrin conduits, fibrin conduits seeded with primary Schwann cells, and fibrin conduits seeded with Schwann cell-like differentiated mesenchymal or adipose-derived stem cells (dMSCs and dASCs). Controls were represented by autografts and by sham rats (tot n=34). At 16 weeks post-implantation, regeneration pattern was analysed on histological sections and related to eventual autophagy. Hindlimbs were evaluated and scored according to autophagy Wall's scale and X-Rays radiological evaluation. RESULTS: All regenerative cell lines significantly improved myelination at the mid conduit level, compared to the empty tubes. However, dMSC could not significantly improve myelination at the distal stump, showing a more chaotic regeneration compared to both other cells groups and controls. Autophagy was correlated to this regeneration patterns, with higher autophagy scores in the empty and dMSC group. CONCLUSION: Hindlimb autophagy can be used as index of neuropathic pain due to nerve lesion or on-going immature regeneration. dMSC group was characterized by a less targeted regeneration comparing to dASC and primary Schwann cells, which confirmed their effectiveness in regeneration and potential in future clinical applications.
INTRODUCTION: Hindlimb autophagy is common after rat sciatic total axotomy and is considered as a sign of neuropathic pain. We applied adult stem cells in a fibrin conduit in a total sciatic axotomy model to improve nerve regeneration, investigating whether a correlation could be detected between stem cells effects on regeneration and limb autophagy. MATERIAL AND METHODS: After sciatic nerve section, a 1-cm sciatic gap was crossed using fibrin conduits. Experimental groups included empty fibrin conduits, fibrin conduits seeded with primary Schwann cells, and fibrin conduits seeded with Schwann cell-like differentiated mesenchymal or adipose-derived stem cells (dMSCs and dASCs). Controls were represented by autografts and by sham rats (tot n=34). At 16 weeks post-implantation, regeneration pattern was analysed on histological sections and related to eventual autophagy. Hindlimbs were evaluated and scored according to autophagy Wall's scale and X-Rays radiological evaluation. RESULTS: All regenerative cell lines significantly improved myelination at the mid conduit level, compared to the empty tubes. However, dMSC could not significantly improve myelination at the distal stump, showing a more chaotic regeneration compared to both other cells groups and controls. Autophagy was correlated to this regeneration patterns, with higher autophagy scores in the empty and dMSC group. CONCLUSION: Hindlimb autophagy can be used as index of neuropathic pain due to nerve lesion or on-going immature regeneration. dMSC group was characterized by a less targeted regeneration comparing to dASC and primary Schwann cells, which confirmed their effectiveness in regeneration and potential in future clinical applications.
Authors: Mario Alessandri-Bonetti; Francesco M Egro; Paolo Persichetti; Sydney R Coleman; J Peter Rubin Journal: Plast Reconstr Surg Glob Open Date: 2019-05-21
Authors: Vânia Tognon-Miguel; Adriana H Nascimento-Elias; Maria C L Schiavoni; Amilton A Barreira Journal: Plast Reconstr Surg Glob Open Date: 2019-01-09
Authors: A G Velichanskaya; D A Abrosimov; M L Bugrova; A V Kazakov; E V Pogadaeva; A M Radaev; N V Blagova; T I Vasyagina; I L Ermolin Journal: Sovrem Tekhnologii Med Date: 2020-10-28