Davide Croci1,2, Edin Nevzati3,4, Hiroki Danura3, Salome Schöpf3, Javier Fandino3,4, Serge Marbacher3,4, Carl Muroi3,4. 1. Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland - neurosurgery@ksa.ch. 2. Neuro Lab, Research Group for Experimental Neurosurgery and Neurocritical Care, Department of Intensive Care Medicine, University Hospital and University of Bern, Bern, Switzerland - neurosurgery@ksa.ch. 3. Department of Neurosurgery, Kantonsspital Aarau, Aarau, Switzerland. 4. Neuro Lab, Research Group for Experimental Neurosurgery and Neurocritical Care, Department of Intensive Care Medicine, University Hospital and University of Bern, Bern, Switzerland.
Abstract
BACKGROUND: In subarachnoid hemorrhage (SAH), occurrence of cerebral vasospasm (CVS) mediated by endothelin (ET)-1 might be a result of a compartmental inflammatory response with interleukin (IL)-6 release. We aim to investigate the relationship between ET-1 and IL-6 in association of CVS. METHODS: A total of 24 New Zealand white rabbits where randomly allocated into 3 groups: SAH (N.=10), IL-6 (N.=10), and sham (N.=4). SAH was induced by a closed cranium extracranial-intracranial shunt model. In the IL-6 group, IL-6 was injected into the cisterna magna. CVS of the basilar artery was assessed by digital subtraction angiography. IL-6 and ET-1 concentrations were measured using enzyme-linked immunosorbent assay. Neuronal damage was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling. RESULTS: A significant increase between baseline (day 0) and follow-up (day 3) was found in CSF IL-6 levels of animals in the SAH and IL-6 group. There was a statistically significant correlation between IL-6 and ET-1 levels in the CSF (Pearson's r=0.454, P=0.003). CVS at day 3 was more pronounced in the SAH than in the IL-6 group: 26.0 ±7.2 % and 16.7 ±5.0 % respectively. TUNEL positive apoptotic neurons in the hippocampal formation were present in the SAH group and in a lesser degree in the IL-6 group. CONCLUSIONS: The results indicate that IL-6 triggered CVS after SAH is ET-1 dependent. IL-6 may be a target for new therapeutic approaches.
BACKGROUND: In subarachnoid hemorrhage (SAH), occurrence of cerebral vasospasm (CVS) mediated by endothelin (ET)-1 might be a result of a compartmental inflammatory response with interleukin (IL)-6 release. We aim to investigate the relationship between ET-1 and IL-6 in association of CVS. METHODS: A total of 24 New Zealand white rabbits where randomly allocated into 3 groups: SAH (N.=10), IL-6 (N.=10), and sham (N.=4). SAH was induced by a closed cranium extracranial-intracranial shunt model. In the IL-6 group, IL-6 was injected into the cisterna magna. CVS of the basilar artery was assessed by digital subtraction angiography. IL-6 and ET-1 concentrations were measured using enzyme-linked immunosorbent assay. Neuronal damage was assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling. RESULTS: A significant increase between baseline (day 0) and follow-up (day 3) was found in CSF IL-6 levels of animals in the SAH and IL-6 group. There was a statistically significant correlation between IL-6 and ET-1 levels in the CSF (Pearson's r=0.454, P=0.003). CVS at day 3 was more pronounced in the SAH than in the IL-6 group: 26.0 ±7.2 % and 16.7 ±5.0 % respectively. TUNEL positive apoptotic neurons in the hippocampal formation were present in the SAH group and in a lesser degree in the IL-6 group. CONCLUSIONS: The results indicate that IL-6 triggered CVS after SAH is ET-1 dependent. IL-6 may be a target for new therapeutic approaches.
Authors: Davide M Croci; Stefan Wanderer; Fabio Strange; Basil E Grüter; Sivani Sivanrupan; Lukas Andereggen; Daniela Casoni; Michael von Gunten; Hans Rudolf Widmer; Stefano Di Santo; Javier Fandino; Luigi Mariani; Serge Marbacher Journal: Transl Stroke Res Date: 2021-01-06 Impact factor: 6.829
Authors: Davide Marco Croci; Stefan Wanderer; Fabio Strange; Basil E Grüter; Daniela Casoni; Sivani Sivanrupan; Hans Rudolf Widmer; Stefano Di Santo; Javier Fandino; Luigi Mariani; Serge Marbacher Journal: Brain Sci Date: 2019-09-24
Authors: Brandon Lucke-Wold; William Dodd; Kartik Motwani; Koji Hosaka; Dimitri Laurent; Melanie Martinez; Victoria Dugan; Nohra Chalouhi; Noelle Lucke-Wold; Arnav Barpujari; Christina von Roemeling; Chenglong Li; Richard D Johnson; Brian Hoh Journal: J Neuroinflammation Date: 2022-09-16 Impact factor: 9.587