PURPOSE: To investigate the effects of human breast milk on corneal epithelial wound healing. METHODS: The effects of human breast milk on epithelial healing is compared with autologous serum and artificial tears on 24 female Bal-b/C mice. A central corneal epithelial defect was created using a 2 mm trephine. Four groups were formed. By a random pick-up, topical human breast milk 4 × 1 was given to Group 1, topical mouse autologous serum 4 × 1 was applied to Group 2, and preservative-free artificial tears 4 × 1 was applied to Group 3.Group 4 was evaluated as control. Biomicroscopical examination was performed on days 1, 2, and 3. Mice were sacrificed on the third day. Histopathological and electron microscopic examinations were performed as well. RESULTS: The fastest and best healing group was Group 1, followed by Group 2. Re-epithelization was not complete even at the end of the second day in groups 3 and 4. CONCLUSIONS: The rich content of human breast milk may be an alternative to epithelial healers and artificial tears.
PURPOSE: To investigate the effects of human breast milk on corneal epithelial wound healing. METHODS: The effects of human breast milk on epithelial healing is compared with autologous serum and artificial tears on 24 female Bal-b/C mice. A central corneal epithelial defect was created using a 2 mm trephine. Four groups were formed. By a random pick-up, topical human breast milk 4 × 1 was given to Group 1, topical mouse autologous serum 4 × 1 was applied to Group 2, and preservative-free artificial tears 4 × 1 was applied to Group 3.Group 4 was evaluated as control. Biomicroscopical examination was performed on days 1, 2, and 3. Mice were sacrificed on the third day. Histopathological and electron microscopic examinations were performed as well. RESULTS: The fastest and best healing group was Group 1, followed by Group 2. Re-epithelization was not complete even at the end of the second day in groups 3 and 4. CONCLUSIONS: The rich content of human breast milk may be an alternative to epithelial healers and artificial tears.
Entities:
Keywords:
Artificial tears; autologous serum eye drops; corneal epithelium; corneal wound healing; human breast milk
Authors: Blanca Espina-Jerez; Laura Romera-Álvarez; Mercedes de Dios-Aguado; Aliete Cunha-Oliveira; José Siles-Gonzalez; Sagrario Gómez-Cantarino Journal: Int J Environ Res Public Health Date: 2022-08-08 Impact factor: 4.614