Jianwen Guo 1 , Lianfu Zuo 1 , Jianghui Liu 1 , Xiaodong Hu 1 , Chunyun Li 1 , Yajuan Zhao 1 , Li Gao 1 Show Affiliations »
Abstract
BACKGROUND: The venom of spiders may inspire new drugs to treat carcinoma. The aim of this study was to investigate the effect of P38 MAPK on tumor necrosis factor-related apoptosis of lung adenocarcinoma cell line by spider venom. METHODS: Lung adenocarcinoma A549 cells were cultured in PRMI-1640 medium and the apoptosis rate was observed after treatment with spider venom with a flow cytometer. The expression of P38 MAPK protein in lung cancer cells was analyzed using Western blot. RESULTS: After targeting spider venom intervention, the expression of P38 MAPK in the cells compared to the control group was significantly down-regulated. The anticancer potency of spider venom is associated with decreased expression of P38 MAPK of lung adenocarcinoma cancer cells. © Tianjin Lung Cancer Institute and Blackwell Publishing Asia Pty. Ltd.
BACKGROUND: The venom of spiders may inspire new drugs to treat carcinoma . The aim of this study was to investigate the effect of P38 MAPK on tumor necrosis factor-related apoptosis of lung adenocarcinoma cell line by spider venom. METHODS: Lung adenocarcinoma A549 cells were cultured in PRMI-1640 medium and the apoptosis rate was observed after treatment with spider venom with a flow cytometer. The expression of P38 MAPK protein in lung cancer cells was analyzed using Western blot. RESULTS: After targeting spider venom intervention, the expression of P38 MAPK in the cells compared to the control group was significantly down-regulated. The anticancer potency of spider venom is associated with decreased expression of P38 MAPK of lung adenocarcinoma cancer cells. © Tianjin Lung Cancer Institute and Blackwell Publishing Asia Pty. Ltd.
Entities: Chemical
Disease
Species
Keywords:
A549 cells; P38 MAPK; anti-cancer drug; spider venom
Year: 2010
PMID： 27755779 DOI： 10.1111/j.1759-7714.2010.00009.x
Source DB: PubMed Journal: Thorac Cancer ISSN： 1759-7706 Impact factor: 3.500