| Literature DB >> 27753034 |
Svetlana N Tamkovich1,2,3, Oleg S Tutanov4, Danil S Serdukov5, Maxim S Belenikin6, Anatoliy G Shlikht7, Natalia A Kirushina8, Vladimir E Voytsitskiy8, Yuri P Tsentalovich9, Vsevolod A Tkachuk10, Pavel P Laktionov4,11.
Abstract
In the current study we have investigated the protein content of blood plasma deoxyribonucleoprotein complexes. The complexes were isolated using affinity chromatography with immobilized polyclonal anti-histone antibodies. Proteins were separated by SDS PAAGE and identified by MALDI-TOF mass-spectrometry. 111 and 56 proteins (excluding histones), respectively, were identified with a good score in deoxyribonucleoprotein complexes of healthy females and breast cancer patients. However, only four of these proteins were found in 30 % of all samples. Fourteen proteins previously described as tumor specific proteins were found in cancer patients whereas not one of them was found in healthy individuals. The data obtained demonstrate the involvement of different cellular and extracellular proteins in circulating cell-free DNA.Entities:
Keywords: Circulating DNA; DNA-binding proteins; MALDI TOF mass spectrometry; Nucleoprotein complexes; Plasma
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Year: 2016 PMID： 27753034 DOI： 10.1007/978-3-319-42044-8_26
Source DB: PubMed Journal: Adv Exp Med Biol ISSN： 0065-2598 Impact factor: 2.622