The ameliorative potential of ethanolic extract of propolis on hematotoxicity and structural neuronal damage in hyperthermia-exposed rats.

OBJECTIVES: Hyperthermia is one of the most common environmental stressors that affect multi-biological systems in the body including the central nervous system as well as the hematopoietic organs. The objective of the present study was to investigate the protective role of ethanolic extract of propolis (EEP) on some selective stress markers, hematological, biochemical, and histopathological changes in rats subjected to hyperthermia (40 °C/12 hr).
MATERIALS AND METHODS: The experimental groups (10 rats each) were classified as follows; Group A; control, (C), was kept at a controlled room temperature (25±5 °C). Group B; ethanolic extract of propolis, (EEP), was fed a basal diet supplemented with 3 g EEP/kg diet for 10 days. Group C; heat stress, (HS), was fed a basal diet for 10 days, and then exposed to high temperature (40±1 °C) for 12 hr. Group D; co-exposed, (EEP+HS) was fed a basal diet supplemented with 3 g EEP/kg diet for 10 days, and then subjected to high temperature (40±1 °C) for 12 hr. At the end of the experimental period, animals were decapitated; blood and tissue samples (brain and spleen) were collected for hematological, biochemical, and histopathological examination.
RESULTS: EEP at a dose of 3 g/kg diet has a potent protective effect against hematotoxicity and brain damage as well as oxidative stress induced by heat stress in rats.
CONCLUSION: The present study indicates that pre-treatment with EEP protects from hematotoxicity and neurological damage induced by high environmental temperature.

Introduction

Recently, many deaths due to hyperthermia were recorded, and all over the world heat-related illnesses in the human population produce serious medical problems (1). Heatstroke is defined as a form of excessive hyperthermia associated with a systemic inflammatory response that results in multiple organ dysfunctions in which central nervous system disorders such as delirium, convulsions, and coma are predominant (2). HS-induced deaths are increasing with global warming and with a worldwide increase in the frequency and intensity of heat waves (3). Nowadays, oxidative stress attracts the attention of many researchers. Heat stress is one of the environmental phenomena that lead to reactive oxygen species (ROS) activation (4). Animal studies suggested that exposure to high temperature (more than 42°C) leads to brain damage through increased free radical production, causing an elevation of lipid peroxidation and limitation of the antioxidant enzymes activity in the brain tissue (5). Erythrocytes are constantly used for oxidative stress evaluation, due to the presence of polyunsaturated fatty acids in their membranes which are the main target for free radical reaction and are very sensitive to lipid peroxidation that results in membrane fluid loss and cellular lysis inducing hemolysis (6, 7).

Propolis is a natural plant product derived from numerous plant resins collected by honey bees. It has been used as a folk medicine for centuries. Propolis has been recorded to exhibit many properties including anticancer (8), antimicrobial, antioxidant, anti-inflammatory (9), antibacterial, antifungal (10), antiviral (11), hepatoprotective (12), and nephropro-tective (13) properties. It has been used as a protective agent against lead-induced neurotoxicity in an animal model (14). Propolis has a protective role in chlorpyrifos-induced changes in the hematological parameters and the oxidative/antioxidative status of Cyprinus carpio carpio (15). Propolis can be effective in the prevention of cypermethrin (CYP)-induced toxicity in rainbow trout, especially hematopoiesis (16). EEP at the supplemented dose of 3 g/kg diet might be considered to prevent oxidative stress in the broilers exposed to heat stress (17). From all of the above, we aspire to focus on the harmful effects resulting from exposure to heat, which lead to hematopoietic disorders and brain damage as a result of oxidative stress.

Materials and Methods

Tested substances and chemicals

Ethanolic extract of propolis 70% (EEP) (Dosic Imp and Exp.Co. Ltd, China) was used in the present experiment. All reagents and chemicals were used of analytical grade purchased from Sigma-Aldrich Co St Louis, MO, USA.

Ethical circumstance

All animals were treated in accordance with the guide for the care and use of laboratory animals (National Institute of Health Publication) and were approved by the Research and Ethics Committee of Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

Heat stress (HS) exposure procedure

Heat stressed rats were placed in an insulated wooden box warmed by a thermostatically controlled infra-red electric lamp (E27, R125 HAOBANG, China) for 12 hr. The box temperature was kept at 40 °C (18). During this time, up to 40 °C rectal temperature was recorded by thermometer.

Animal grouping and experimental design

Forty adult male albino rats (3 months old, weighing 220±10 g) were purchased from the Laboratory Animal Breeding Unit, Faculty of Veterinary Medicine, Zagazig University. Rats were acclimatized for one week prior to the beginning of the experimental study. The animals were housed in an insulated wooden box in a temperature-controlled room (25±5°C) with relative humidity (50±10) and with 12 hr light/dark cycle. Rats were allowed a standard commercial chow diet.

This study was carried out on 40 adult male albino rats, divided into four main groups (n=10);

Group A (C) was kept at a controlled room temperature (25±5°C).

Group B (EEP) was fed a basal diet supplemented with 3 g EEP/kg diet for 10 days (17).

Group C (HS) was fed a basal diet for 10 days and then exposed to high temperature (40±1°C) for 12 hr.

Group D (EEP+HS) was fed a basal diet supplemented with 3 g EEP/kg diet for 10 days and then subjected to high temperature (40±1°C) for 12 hr.

Sampling

Animals were fasted overnight, decapitated, and sacrificed for obtaining the blood and tissue samples. Whole blood was used for hematological analysis, serum was collected for serum iron, total, direct, and indirect bilirubin meanwhile, the collected plasma was used for determination of malondialdehyde (MDA), noradrenaline (NA), adrenaline (A), corticosterone (Cs), and glucose levels while the sedimented erythrocytes were washed three times with saline 0.9 and lysed with distilled water (1:3, v/v) at 0°C for 30 min then extracted from the lysate and stored at -80°C until used for measuring the reduced glutathione (GSH) level. The brain was removed from the skull and rinsed with sterile physiological saline (0.9%, NaCl) and cut into two equal parts; the first part was homogenized in 5 ml of cold phosphate buffer saline (pH 7.4) by Universal Laboratory Aid Homogenizer (MPW-309, Mechanika Precyzyjna, Warsaw, Poland). Homogenates were centrifuged using BOECO centrifuge (Germany) at 3000 rpm for 15 min at 4°C. The supernatants were collected and frozen at −20°C until used for assaying of caspase-3, Bcl-2, dopamine, serotonin, gamma-aminobutyric acid (GABA), MDA, and GSH, meanwhile, the second part of the brain and additional tissue fragments from spleen were fixed with neutral buffered formalin 10% for histopathological examination.

Hematological evaluation

Total erythrocytes counts (TEC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration (MCHC) were evaluated by using an automated hematology analyzer (Hospitex Hema Screen 18 analyzer, Italy) (19). Reticulocytes (retics) were counted on blood smear stained with new methylene blue stain (20). Retics number was expressed in percentage (number of reticulocytes per total number of RBC x 100%).

Estimation of hemolysis markers

Serum iron was analyzed colorimetrically using SGM kits according to the methods of Ruutu (21). Spleen iron was measured colorimetrically after acid digestion of tissues (22). The serum bilirubin levels (total and direct) were assayed by using a commercial kit obtained from Diamond Diagnostics, Egypt accor-ding to Jendrassik (23). Indirect bilirubin was calculated by subtracting the obtained direct bilirubin level from the total bilirubin level.

Estimation of stress biomarkers and neurotrans-mitters

Plasma noradrenaline (NA) and adrenaline (A) concentrations were measured by using commercial radioimmunoassay (RIA) kits (IBL, Germany) following the instructions of the manufacturer. Corticosterone level was determined by the method of Vazquez-Palacios (24) using ELISA kit (DRG instruments GmbH, Germany). Moreover, glucose level was estimated by glucose oxidase–peroxidase method of Trinder (25) using a colorimetric kit of Biodiagnostics, Egypt. Sero-tonin, dopamine, and GABA levels were measured according to the methods described by Hou; Ciarlone, and Seiler, respectively (26-28).

Parameters related to DNA damage and apoptosis assaying

Brain caspase-3 was measured according to Fernandes-Alnemri et al (29). The Bcl-2 protein level was determined by ELISA kits of Uscn Life Science Inc., the procedure was carried out through the enclosed pamphlet method.

Oxidative stress indices

Total proteins in brain homogenate were measured colorimetrically according to Lowry et al (30). Brain homogenate MDA and GSH were determined according to the methods described previously by Ohkawa et al and Beutler et al, respectively (31, 32). Meanwhile, in blood, malondialdehyde, and reduced glutathione levels were estimated using the methods of Ohkawa et al and Beutler, respectively (31, 33).

Histopathological investigation

Brain and spleen specimens were obtained, fixed in neutral buffered formalin 10% for 7 days. The formalin-fixed samples were continuously transferred to freshly prepared fixative. The preserved samples were briefly dehydrated in a graded series of ethanol, cleared in 3 changes of xylene and embedded in paraffin wax. Paraffin blocks were sectioned into 4–5 μm thick sections. Finally, the paraffin sections were subjected to hematoxylin and eosin stain (H&E) according to Bancroft and Gamble (34). Histological sections were viewed and representative photomicrographs were taken using Olympus BX41 research optical photomicroscope equipped with an Olympus DP25 digital camera, courtesy Cytology and Histology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. Histopathological scoring was microscopically done and scored blindly in a table.

Statistical analysis

Data were analyzed by means of one-way (ANOVA) using SPSS statistical software (ver. 16.00, USA). Data are expressed as the mean±SE, and the results were statistically significant at P<0.05 (35).

Results

Effect of heat stress and EEP co-exposure on erythrogram

Basic statistics for the erythrogram of different experimental groups were shown in Table 1. Hemolytic anemia could be easily observed in the heat stressed animals showing macrocytosis and hypochromasia with reticulocytosis while pretreatment with EEP improves the erythrogram picture.

Table 1

Erythrogram variables in different experimental groups

Treatments
Parameters	C	EEP	HS	EEP + HS
TEC (106/mm3)	6.22± 0.32a	6.07± 0.54a	4.09±0.32b	5.13± 0.19ab
Hb (gm/dl)	16.00± 0.23a	15.11± 1.06a	10.60± 0.30b	12.60± 0.61b
Ht (%)	39.60± 0.83a	38.60± 1.80ab	31.06± 0.40c	35.00±1.73bc
MCV (fl)	63.89± 2.22b	64.07± 3.07b	76.60± 4.96a	68.09±1.31ab
MCHC (%)	40.41± 0.32a	39.06± 0.98a	34.10± 0.61b	36.01± 0.57b
Retics (%)	2.56± 0.23c	2.46± 0.24c	5.63± 0.34a	4.03± 0.14b

C: Control; EEP: ethanolic extract of propolis; HS: heat stress; TEC: Total erythrocytes counts; Hb: Hemoglobin; Ht: Hematocrit; MCV: Mean corpuscular volume; MCHC: Mean corpuscular hemoglobin concentration

Data are expressed as the mean±SE. Means within the same row carrying different superscripts are significantly different (one-way ANOVA followed by Duncan’s multiple range test, P<0.05, n=5 /group)

HS and/or EEP exposure induced oxidative hemolytic changes

Concerning the erythrocytes oxidative stress and hemolysis features, Table 2 showed a significant (P< 0.05) increase in MDA level, serum and spleen iron as well as total and indirect bilirubin with a significant (P<0.05) decrease in erythrocyte GSH contents in animals exposed to high temperature (40 °C/12 hr) compared to the control rats. Meanwhile, all these parameters were improved in animals pre-treated with ethanolic extract of propolis at a dose of 3 g EEP/kg diet for 10 days prior to heat exposure but were not returned to the control level.

Table 2

Effect of heat stress exposure and/or ethanolic extract of propolis on blood oxidative and hemolysis indices in rats

Treatments
Parameters	C	EEP	HS	EEP + HS
GSH (nmol/ml)	1147.07±7.66a	1155.50±6.64a	1023.49±11.33b	1103.87±6.61c
MDA (nmol/ml)	3.27±0.17c	3.13±0.14c	12.31±0.36a	6.49±0.39b
Serum Fe (µmol/l)	15.82±0.15c	15.99±0.35c	36.06±0.38a	23.82±0.81b
Spleen Fe (µg Fe/g)	45.78±0.50c	45.65±0.97c	65.63±0.29a	54.20±0.48b
T.bilirubin (mg%)	1.61±0.11c	1.64±0.18c	4.49±0.22a	2.89±0.08b
D.bilirubin (mg%)	0.40±0.03a	0.37±0.03a	0.36±0.02a	0.36±0.02a

C: Control; EEP: ethanolic extract of propolis; HS: heat stress; GSH: Reduced glutathione; MDA: Malondialdehyde; Fe:Iron; T.bilirubin: Total bilirubin; D.bilirubin: Direct bilirubin.

Data are expressed as the mean±SE. Means within same row carrying different superscripts are significantly different (one-way ANOVA followed by Duncan’s mcultiple range test, P<0.05, n= 5 /group)

Stress biomarkers alterations following HS and/or EEP exposure

Regarding the biochemical analysis of selective stress biomarkers including, corticosterone, adrenaline, noradrenaline, and glucose showed a significant increase in animals acutely exposed to high temperature compared with control ones. Addition of EEP to diet prior to high-temperature exposure resulted in marked (P<0.05) reduction in the above-mentioned stress markers compared with the HS group but not return to the normal control values as shown in Table 3.

Table 3

Effect of heat stress exposure and/or ethanolic extract of propolis on stress markers level in rats

Treatments
Parameters	C	EEP	HS	EEP+HS
Corticosteroe (µg/dl)	64.88±1.28c	63.04±1.16c	117.23±3.29a	92.59±1.36b
Adrenaline (pg/ml)	425.59±2.20c	428.87±0.70c	833.1±25.51a	600.7±5.34b
Noradrenalie (pg/ml)	1141.85±17.66c	1138.0±12.4c	1667.6±23.7a	1321.1±21.4b
Glucose	103.22±2.24c	104.15±1.70c	272.76±2.27a	179.2±1.56b
(mg/dl)

C: Control; EEP: ethanolic extract of propolis; HS: heat stress

Data are expressed as the mean±SE. Means within same row carrying different superscripts are significantly different (one-way ANOVA followed by Duncan’s multiple range test, P<0.05, n=5/group

Oxidative, apoptotic brain injury following HS and/or EEP exposure

As shown in Table 4, a significant increase in brain dopamine, serotonin, and caspase-3 contents was found in addition to a marked decrease in brain GABA; BCL-2 also was noticed in heat stressed rats compared to the control group. On the other hand, pre-treatment with propolis ameliorates the level of these parameters near to the control group compared with the HS group.

Table 4

Effect of heat stress and/or EEP on neurotransmitters, apoptotic, and oxidative stress indices in rats

Treatments
Parameters	C	EEP	HS	EEP+HS
Neurotransmitters
Dopamine (µg/g tissue)	17.26± 0.33c	17.21±0 .78c	29.26 ±1.11a	21.81 ±1.38b
Serotonin (µg/g tissue)	6.36±0.33c	6.19± 0.40c	16.36 ±0.33a	11.64± 0.69b
GABA (µg/g tissue)	99.83± 0.54a	100.27± .65a	71.02 ±2.07c	92.77 ± 1.13b
Apoptosis markers
Caspase-3 (ng/100mg)	119.14 ±0.60c	118.60±0.74c	164.74±7.20a	135.81±2.90b
BCL-2 (ng/mgtissue)	74.82± 5.46a	72.06± 2.04a	42.98± 1.12c	60.04± 3.33b
Oxidative stress markers
GSH (nmol/mg protein)	30.35±0.25a	30.85±0.12a	15.96±0.21c	29.12±0.13b
MDA(nmol/mg protein)	0.71±0.02c	0.69±0.04c	1.38±0.01a	0.94±0.02b

Data are expressed as the mean±SE. Means within same row carrying different superscripts are significantly different (one-way

ANOVA followed by Duncan’s multiple range test, P<0.05, n=5/group); C: Control; EEP: ethanolic extract of propolis; HS: heat stress

Also, a significant (P< 0.05) increase in MDA level with a decrease in GSH content was found in brain homogenate of animals exposed to high temperature compared to the control rats. Observable reduction in MDA level and elevation in GSH content close to the control level were noticed in animals supplemented with ethanolic extract of propolis at a dose of 3 g EEP/kg diet for 10 days prior to acute heat exposure compared to the heat exposed animals.

Histopathological findings following HS and/or EEP exposure

The severity of alterations in the brain and spleen specimens of different experimental groups was blindly scored microscopically, and the scores are presented in Table 5.

Table 5

Scoring of the histopathological alterations in brain and spleen sections of rats of different treated groups

Lesions	Treatments
	C	EEP	HS	EEP +HS
Brain tissue
Meningeal cellular proliferation	--	--	+++	+
Neuronal degeneration	--	--	++	+
Focal gliosis	--	--	++	--
Congested blood vessels	--	--	++	--
Submeningeal hemorrhage	--	--	+	--
Spleen tissue
Activated lymphoid follicles	--	+	--	--
Congested blood vessels	--	--	++	+
Lymphocytic depletion	--	--	++	--
Hemosiderosis	--	--	+++	+

C: Control; EEP: ethanolic extract of propolis; HS: heat stress

+++ : Severe histopathological alteration

++ : Moderate histopathological alteration

+ : Mild histopathological alteration

-- : Nil histopathological alteration

Microscopical examination of H&E stained brain sections of control animals showing normal cerebral architecture with different sized intact basophilic neurons and randomly oriented axonal fibers, normally distributed neuroglia cells and intact meningeal cell layers (Figure 1 a). High magnification of the previous figure focused on the intact basophilic neurons (Figure 1 b). The propolis-treated group showed normal histological cerebral architecture (Figure 1 c). Meanwhile, the brain of heat stressed animals’ revealed meningeal cellular proliferation with congested blood vessels and mild submeningeal hemorrhage (Figure 1 d).

Figure 1

Photomicrographs of the H&E stained brain sections of the control group (C) showing normal cerebral architecture with different sized intact basophilic neurons (black arrows) and randomly oriented axonal fibers (white arrows), normally distributed neuroglia cells (head arrows) and intact meningeal cell layers (thick arrow) (a). High magnification of the previous figure focused on the intact basophilic neurons (b). Propolis administered group (EEP) showing normal histological cerebral architecture (c). Heat stressed group (HS) showing meningeal cellular proliferation (P) with congested blood vessels (C) and mild submeningeal hemorrhage (H) (d), additional extravasated blood cells (E) were also observed between the neuronal tissues (e) with evidence of focal gliosis (G) detected by occasional aggregation of small rounded deeply basophilic neuroglia cells (f). Neuronal degeneration was observed and characterized by swollen rounded vacuolated neurons with pyknotic nuclei (white arrow) (g). The heat stressed group treated with propolis (EEP + HS) showing normal intact meninges and absence of both hemorrhage and gliosis with few to moderate numbers of neurons showing mild degenerative changes with occasional swelling and vacuolation (h)

Extravasated blood cells were also observed between the neuronal tissues (Figure 1 e). Evidence of focal gliosis was detected by occasional aggregation of small rounded deeply basophilic neuroglia cells (Figure 1 f). Neuronal degeneration was observed and characterized by swollen rounded vacuolated neurons with pyknotic nuclei (Figure 1 g). Regarding heat stressed groups treated with propolis, the neuronal tissues showed normal intact meninges and absence of both hemorrhage and gliosis.

Few to moderate numbers of neurons showed mild degenerative changes with occasional swelling and vacuolation (Figure 1 h).

Spleen of the control group showed a normal splenic structure with normal white and red pulp integrity and absence of hemosiderosis (Figure 2 a). The propolis administered group (EEP) showing normal histological splenic architecture as control one with active lymphoid follicles (Figure 2 b). Heat stressed group showing congested blood vessels and sinusoids among the red pulp (Figure 2 c) along with hemosiderosis was observed as yellowish brown pigment inside the phagocytic cells (Figure 2 d). Moderate to marked lymphocytic depletion was represented by smaller lymphatic nodules with occasional degeneration and necrosis of lymphocytes especially at the rim of lymphoid follicles (Figure 2 e). The co-exposed group showing significant improvement of the splenic architecture with mildly congested blood vessels and slight yellowish-brown pigmentation (Figure 2 f).

Figure 2

Photomicrographs of the H&E stained spleen of the control group (C) showing a normal splenic structure with normal white (W) and red pulp (R) integrity and absence of hemosiderosis (a). Propolis administered group (EEP) showing normal histological splenic architecture as control one with active lymphoid (L) follicles (b). Heat stressed group (HS) showing congested (C) blood vessels and sinusoids among the red pulp (c), along with hemosiderosis was observed as yellowish brown pigment inside the phagocytic cells (white arrows) (d). Moderate to marked lymphocytic depletion (LD) was represented by smaller lymphatic nodules with occasional degeneration and necrosis of lymphocytes especially at the rim of lymphoid follicles (e). The co-exposed group showing significant improvement of the splenic architecture with mildly congested blood vessels and slight yellowish-brown pigmentation (f)

Discussion

Studies on the heat stress induced alterations are still increasing, as temperature is one of the most encountered stressful factors in different biological systems. Heat stress (HS) is considered a model of thermal injury to the central nervous system (CNS) (36).

Hyperthermia as a result of high summer heat exposure was found to be one of the most common environmental stressors that induce excess ROS production (37), causing oxidative damage to many vital organs, especially the brain, leading to neurodegenerative disorder (38) and consequently decline in the animal’s performance (39).

Upon exposure to higher temperatures in the present work, an increase in oxidative damage markers such as MDA combined with a reduction in GSH in brain homogenate resulted in brain damage was recorded. The presented data in this study agreed with researchers (40) who found that heat acclimation (HA) on acute exhaustive exercise-rats model induced oxidative stress (OxS) and inflammatory reactions. These results were confirmed by the histopathological findings which revealed meningeal cellular proliferation with congested blood vessels, mild submeningeal hemorrhage, extravasated blood cells between the neuronal tissues, focal gliosis and neuronal degeneration in the heat stressed group. These findings were previously obtained by Boulant (41) who reported that rats with hyperpyrexia showed local hemorrhages and parenchymatous degeneration of brain cells. Research (42) found that brains of heat exposed rats (40°C /12 hr) show hyperemic capillaries and blood vessels and focal hemorrhage in cerebral tissue.

Heat stroke influences excitatory and inhibitory amino acid neurotransmitters in the central nervous system. Our results showed a significant increase in adrenaline, noradrenaline, corticosterone, glucose, dopamine, and serotonin as stress markers. Increased levels of these neurotransmitters could be attributed to their great role in temperature regulation. Noradrenaline and adrenaline are chemical transmitters at most sympathetic postganglionic endings, stored in the synaptic knobs of adrenergic neurons. Corticosterone is the terminal product of the HPA axis and one of the adrenal cortical synthetic glucocorticoids (43). Dopamine is the noradrenaline precursor found in the dopaminergic neurons of brain hypothalamus. Serotonin is a synaptic mediator formed from tryptophan hydroxylation and decarboxylation. Our results appeared to be parallel with literature (42, 44, 45). GABA is a non-protein amino acid acting as a signaling molecule that helps in the regulation of many stress responses through controlling the carbon/nitrogen balance, osmotic potential, free radical scavenging, and pH regulation (46, 47). In the present study, the level of GABA significantly decreased under heat stress, this was observed by (1) who found that experimental rats exposed to high temperature (38 °C/4 hr) showed a marked decrease in GABA.

Heat-stress induced apoptosis and necrosis as shown in our work by increasing brain caspase-3 and decreasing Bcl-2 content. Caspase-3 is an essential apoptosis marker; its stimulation is a feature of cellular apoptosis (48), and Bcl2 is one of the antiapoptotic proteins (49). The changes in both can be attributed to oxidative damage induced by heat stress in brain cells leading to its death. Our results agreed with the previous results, which found an elevation of caspase-3 and declining of Bcl-2 in cardiomyocyte (50) and liver cells (51) of heat-stressed animals.

Regarding hematological alterations resulting from exposure to high ambient temperature; we found that heat exposed rats showed hemolytic anemia, which was confirmed by reticulocytosis, increased serum and spleen iron with hyperbilirubinemia and splenic hemosiderosis. These results could be linked to the increased generation of highly free radicals in mitochondria or due to the elevation of erythrocyte osmotic fragility in hot-dry seasons (52). Our results agreed with researchers (53) who reported that heat-stress induced hemolysis in rats during hot-dry seasons with decreased erythrocyte count, hemoglobin concentration, and packed cell volume.

Propolis, a natural therapeutic agent, shows antioxidant activities (54). Propolis extract contains many flavonoids and phenolic compounds which are powerful antioxidants, free radical scavengers, and lipid peroxidation inhibitors. Several studies suggested that propolis and its components protect the brain from ischemia-reperfusion damage through their antioxidant (55) and anti-inflammatory activities (56). In the present work, we found that EEP protects the brain and erythrocyte from oxidative damage induced by heat stress and this was confirmed by normal intact meninges of neuronal tissues with absence of both hemorrhage and gliosis and presence of few to moderate numbers of degenerative neurons.

Conclusion

From the above-mentioned details, we found that ethanolic extract of propolis protects from oxidative hematological alterations and neurological damage induced by high environmental temperature.