Xiu Chen1, Peng Lu2, Dan-Dan Wang3, Su-Jin Yang4, Ying Wu3, Hong-Yu Shen4, Shan-Liang Zhong5, Jian-Hua Zhao6, Jin-Hai Tang7. 1. The Fourth Clinical School of Nanjing Medical University, 210009 Nanjing, China; Department of General Surgery, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Baiziting42, 210009 Nanjing, China; The First Affiliated Hospital of Nanjing Medical University, Guangzhoulu300, 210029 Nanjing, China. 2. School of Public Health Nanjing Medical University, 210009 Nanjing, China. 3. The First Clinical School of Nanjing Medical University, 210009 Nanjing, China. 4. The Fourth Clinical School of Nanjing Medical University, 210009 Nanjing, China. 5. The First Affiliated Hospital of Nanjing Medical University, Guangzhoulu300, 210029 Nanjing, China. 6. Center of Clinical Laboratory Science, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Baiziting42, 210009 Nanjing, China. Electronic address: jsccljhzhao@126.com. 7. Department of General Surgery, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Baiziting42, 210009 Nanjing, China; The First Affiliated Hospital of Nanjing Medical University, Guangzhoulu300, 210029 Nanjing, China. Electronic address: jschjhtang@163.com.
Abstract
PURPOSE: Chemoresistance mediated by miRNAs in breast cancer have been already validated by previous studies in vitro, while little is known concerning the expression of them in vivo. The aim of this study was to investigate the role of miR-222, miR-29a, miR-34a, miR-130a, miR-90b, miR-200b, miR-452, miR-197, miR-138, miR-210, miR-423, miR-4298, miR-4644, miR-139, miR-1246, miR-1268a, miR-140, miR-149, miR-3178, miR-3613, miR-4258, miR-574, miR-671, miR-6780b, miR-7107, miR-744 and miR-7847 linked to drug resistance in breast cancer formalin-fixed paraffin-embedded tissues and the association of prognosis with miRNAs, thus providing effective targets in chemotherapy, as well as potential biomarkers for guiding effective treatments of breast cancer. METHODS: The relationship between the expression of diverse miRNAs and drug resistance was detected by RT-qPCR using 55 breast cancer FFPE tissues containing 26 paired FFPE specimens. RESULTS: MiR-222, miR-29a, miR-34a, miR-423, miR-140, miR-3178, miR-574, miR-6780b and miR-744 exhibited significantly higher expression levels in surgically-resected specimens compared with pre-neoadjuvant chemotherapy biopsies. Evidently high expression of miR-222, miR-29a, miR-140, miR-574, miR-6780b, miR-7107 and miR-744 were found in ineffective group comparing with effective group. Further investigations revealed the significant association between several miRNAs in breast cancer patients. CONCLUSIONS: This study highlights the role of numerous miRNAs in prediction of therapeutic responses and suggests that specific miRNAs could serve as valuable sources for biomarker detections and optimal chemotherapeutic choices for breast cancer patients.
PURPOSE: Chemoresistance mediated by miRNAs in breast cancer have been already validated by previous studies in vitro, while little is known concerning the expression of them in vivo. The aim of this study was to investigate the role of miR-222, miR-29a, miR-34a, miR-130a, miR-90b, miR-200b, miR-452, miR-197, miR-138, miR-210, miR-423, miR-4298, miR-4644, miR-139, miR-1246, miR-1268a, miR-140, miR-149, miR-3178, miR-3613, miR-4258, miR-574, miR-671, miR-6780b, miR-7107, miR-744 and miR-7847 linked to drug resistance in breast cancerformalin-fixed paraffin-embedded tissues and the association of prognosis with miRNAs, thus providing effective targets in chemotherapy, as well as potential biomarkers for guiding effective treatments of breast cancer. METHODS: The relationship between the expression of diverse miRNAs and drug resistance was detected by RT-qPCR using 55 breast cancer FFPE tissues containing 26 paired FFPE specimens. RESULTS:MiR-222, miR-29a, miR-34a, miR-423, miR-140, miR-3178, miR-574, miR-6780b and miR-744 exhibited significantly higher expression levels in surgically-resected specimens compared with pre-neoadjuvant chemotherapy biopsies. Evidently high expression of miR-222, miR-29a, miR-140, miR-574, miR-6780b, miR-7107 and miR-744 were found in ineffective group comparing with effective group. Further investigations revealed the significant association between several miRNAs in breast cancerpatients. CONCLUSIONS: This study highlights the role of numerous miRNAs in prediction of therapeutic responses and suggests that specific miRNAs could serve as valuable sources for biomarker detections and optimal chemotherapeutic choices for breast cancerpatients.