| Literature DB >> 27746242 |
Elena Parra1, Ferran Segura1, Jessica Tijero1, Imma Pons1, Maria-Mercedes Nogueras2.
Abstract
A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng-100 fg and sensitivity was 50 copies/reaction.Entities:
Keywords: Bartonella; Optimization; Real-time PCR; Zoonoses
Mesh：
Year: 2016 PMID： 27746242 DOI： 10.1016/j.mcp.2016.10.006
Source DB: PubMed Journal: Mol Cell Probes ISSN： 0890-8508 Impact factor: 2.365