Xiaodong Cai1,2, Ling Long3, Libai Yang2,4, Ziyi Chen2, Guanzhong Ni2, Jiaming Qin2, Jueqian Zhou2, Liemin Zhou2. 1. a Department of Neurology , the Sixth Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China. 2. b Department of Neurology , the First Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China. 3. c Department of Neurology , the Third Affiliated Hospital of Sun Yat-Sen University , Guangzhou , China. 4. d Department of Neurology , Shanxi Academy of Medical Sciences & Shanxi Dayi Hospital , Taiyuan , China.
Abstract
OBJECTIVES: Mossy fiber sprouting is involved in the pathogenesis of mesial temporal lobe epilepsy. But the exact mechanism of formation of mossy fiber sprouting is still unclear. Semaphorin-3f protein could inhibit the growth of neuron axons. The aim of this research is to evaluate the association between semaphorin-3f expression and mossy fiber sprouting. METHODS: We established pilocarpine-induced status epilepticus (PISE) models firstly. Then, mossy fiber sprouting in the hippocampus of PISE models was examined by Timm staining. Expression of semaphorin-3f was evaluated by western blot analysis and immunohistochemical examination. Expression of semaphorin-3f protein in different subregions of hippocampus and its relationship with mossy fiber sprouting were studied. RESULTS: We found that in PISE group, mossy fiber sprouting appeared in dentate gyrus (DG) region. It started to develop in the latent phase of PISE group and increased significantly in the chronic phase. Expression of semaphorin-3f protein in DG region started to decrease in the latent phase, and stayed at low level in the chronic phase. No such change was found in the other groups. CONCLUSIONS: These results indicate that the decrease in semaphorin-3f expression in DG region was in parallel to the change of mossy fiber sprouting in PISE models, suggesting that mossy fiber sprouting is closely associated with reduced expression of semaphorin-3f in this model.
OBJECTIVES: Mossy fiber sprouting is involved in the pathogenesis of mesial temporal lobe epilepsy. But the exact mechanism of formation of mossy fiber sprouting is still unclear. Semaphorin-3f protein could inhibit the growth of neuron axons. The aim of this research is to evaluate the association between semaphorin-3f expression and mossy fiber sprouting. METHODS: We established pilocarpine-induced status epilepticus (PISE) models firstly. Then, mossy fiber sprouting in the hippocampus of PISE models was examined by Timm staining. Expression of semaphorin-3f was evaluated by western blot analysis and immunohistochemical examination. Expression of semaphorin-3f protein in different subregions of hippocampus and its relationship with mossy fiber sprouting were studied. RESULTS: We found that in PISE group, mossy fiber sprouting appeared in dentate gyrus (DG) region. It started to develop in the latent phase of PISE group and increased significantly in the chronic phase. Expression of semaphorin-3f protein in DG region started to decrease in the latent phase, and stayed at low level in the chronic phase. No such change was found in the other groups. CONCLUSIONS: These results indicate that the decrease in semaphorin-3f expression in DG region was in parallel to the change of mossy fiber sprouting in PISE models, suggesting that mossy fiber sprouting is closely associated with reduced expression of semaphorin-3f in this model.
Authors: Ka Lai Yip; Chi Man Koon; Zi Yi Chen; Ping Chook; Ping Chung Leung; Steven Schachter; Wai Hong Leung; Chung Tong Mok; Howan Leung Journal: Epilepsia Open Date: 2019-12-13