Christelle Pomares1, Tyson H Holmes2, Remy Estran3, Cynthia J Press4, Raymund Ramirez4, Jeanne Talucod4, Holden Maecker5, Yael Rosenberg-Hasson5, Jose G Montoya6. 1. Palo Alto Medical Foundation Toxoplasma Serology Laboratory, Palo Alto, CA, USA; Division of Infectious Diseases, Stanford University, Stanford, CA 94305, USA; INSERM, U1065, Centre Méditerranéen de Médecine Moléculaire, C3M, Toxines Microbiennes dans la Relation Hôte Pathogènes - Université de Nice Sophia Antipolis d, Faculté de Médecine, 06204 Nice Cedex 3, France; Parasitologie-Mycologie, Centre Hospitalier Universitaire l'Archet e, CS 23079, 06202 Nice Cedex 3, France. Electronic address: pomares.c@chu-nice.fr. 2. Stanford University Human Immune Monitoring Centre, Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, CA 94305, USA. 3. ESCP Europe, 75543 Paris Cedex 11, France. 4. Palo Alto Medical Foundation Toxoplasma Serology Laboratory, Palo Alto, CA, USA. 5. Department of Immunology, Fairchild Science Building, D033, 299 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5124, USA. 6. Palo Alto Medical Foundation Toxoplasma Serology Laboratory, Palo Alto, CA, USA; Division of Infectious Diseases, Stanford University, Stanford, CA 94305, USA.
Abstract
INTRODUCTION: Majority of Toxoplasma gondii infections are benign and asymptomatic; however, some patients experience toxoplasmic lymphadenitis (TL). Factors associated as to whether infection will be symptomatic or not are unknown. METHODS: Dye test titers of patients with acute toxoplasmosis (pregnant and not pregnant) with TL (TL+) were compared with those in patients with asymptomatic acute infection (TL-). Additionally, mean levels of 62 serum cytokines were compared between TL+ and TL- pregnant women and between TL+ pregnant and non-pregnant women. RESULTS: During acute infection, mean dye test titer was higher in TL+ than in TL- patients (p=0.021). In addition, out of 62 cytokines, CXCL9andCXCL10 levels were higher (p<0.05) and resistin mean levels were lower (p<0.05) in pregnant women with TL+ compared to TL-. Among patients with TL+, levels of VCAM1andCCL2 were lower (p<0.05) in pregnant women than in non-pregnant women. CONCLUSION: Here we report differences in dye test titers in patients with acute infection. Cytokine responses vary according to the presence of TL+ and to the pregnancy status. Factors underlying these differences are presently unknown and require further studies to define individual and combined roles of cytokines in TL+.
INTRODUCTION: Majority of Toxoplasma gondii infections are benign and asymptomatic; however, some patients experience toxoplasmic lymphadenitis (TL). Factors associated as to whether infection will be symptomatic or not are unknown. METHODS: Dye test titers of patients with acute toxoplasmosis (pregnant and not pregnant) with TL (TL+) were compared with those in patients with asymptomatic acute infection (TL-). Additionally, mean levels of 62 serum cytokines were compared between TL+ and TL- pregnant women and between TL+ pregnant and non-pregnant women. RESULTS: During acute infection, mean dye test titer was higher in TL+ than in TL- patients (p=0.021). In addition, out of 62 cytokines, CXCL9andCXCL10 levels were higher (p<0.05) and resistin mean levels were lower (p<0.05) in pregnant women with TL+ compared to TL-. Among patients with TL+, levels of VCAM1andCCL2 were lower (p<0.05) in pregnant women than in non-pregnant women. CONCLUSION: Here we report differences in dye test titers in patients with acute infection. Cytokine responses vary according to the presence of TL+ and to the pregnancy status. Factors underlying these differences are presently unknown and require further studies to define individual and combined roles of cytokines in TL+.
Authors: Holden T Maecker; Janet C Siebert; Yael Rosenberg-Hasson; Lorrin M Koran; Miguel Ramalho; Richard C Semelka Journal: Invest Radiol Date: 2021-06-01 Impact factor: 10.065