Literature DB >> 2774179

Isoelectric points of proteins: theoretical determination.

A Sillero1, J M Ribeiro.   

Abstract

Three methods for calculating the isoelectric points (pI) of proteins, provided that their amino acid compositions are known, are described. The comprehensive and abridged procedures involve solutions of polynomial equations of different degrees depending on whether pK values of the specific acid-base residues or the means of some of those values, respectively, are adopted. In the simplified procedure, approximate pI values of proteins can be determined easily with the help of calculated values, included in this paper, related to the amino acid composition of proteins.

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Year:  1989        PMID: 2774179     DOI: 10.1016/0003-2697(89)90136-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  30 in total

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Review 4.  The isoelectric point, a key to understanding a variety of biochemical problems: a minireview.

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Journal:  Fish Physiol Biochem       Date:  2007-08-09       Impact factor: 2.794

5.  What drives amyloid molecules to assemble into oligomers and fibrils?

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Journal:  Biophys J       Date:  2011-01-19       Impact factor: 4.033

6.  Soluble FasR ligand-binding domain: high-yield production of active fusion and non-fusion recombinant proteins using the baculovirus/insect cell system.

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7.  Cloning, sequencing, and expression of a xylanase gene from the anaerobic ruminal bacterium Butyrivibrio fibrisolvens.

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Journal:  J Bacteriol       Date:  1990-08       Impact factor: 3.490

8.  Marginal protein stability drives subcellular proteome isoelectric point.

Authors:  Kaiser Loell; Vikas Nanda
Journal:  Proc Natl Acad Sci U S A       Date:  2018-11-01       Impact factor: 11.205

9.  A proteomic study reveals novel insights into the diversity of aquaporin forms expressed in the plasma membrane of plant roots.

Authors:  Véronique Santoni; Joëlle Vinh; Delphine Pflieger; Nicolas Sommerer; Christophe Maurel
Journal:  Biochem J       Date:  2003-07-01       Impact factor: 3.857

10.  Hydrolysis of bis(5'-nucleosidyl) polyphosphates by Escherichia coli 5'-nucleotidase.

Authors:  A Ruiz; C Hurtado; J Meireles Ribeiro; A Sillero; M A Günther Sillero
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

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