Ping Lin1, Bingyu Ding2, Chunyan Feng3, Shuwei Yin4, Ting Zhang4, Xin Qi4, Huiying Lv4, Xiaokui Guo5, Ke Dong5, Yongzhang Zhu5, Qingtian Li6. 1. Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. 2. Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. 3. Department of internal medicine, Zhejiang Medical College, Hangzhou 310053, China. 4. Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. 5. Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. 6. Department of Laboratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: qingtianli@gmail.com.
Abstract
BACKGROUND: The diagnosis of major depression disorder (MDD) and other mental disorders were depended on some subjective survey scales. There are confirmed relationship between the gut flora and the mental states of MDD patients. METHODS: The V3-V4 region of the 16S rRNA gene was extracted from the fecal microbial communities in MDD patients, PCR amplified and sequenced on the Illumina Miseq platform. RESULTS: More phylum Firmicutes, less Bacteroidetes, and more genus Prevotella, Klebsiella, Streptococcus and Clostridium XI were found in MDD patients. The changes of the proportion of Prevotella and Klebsiella were consistent with Hamilton depression rating scale. LIMITATIONS: The conclusion was limited by small sample sizes and potential uncontrollable influence factors on fecal microbiota. DISCUSSION: Prevotella and Klebsiella proportion in fecal microbial communities should be concerned in the diagnosis and therapeutic monitoring of MDD in future.
BACKGROUND: The diagnosis of major depression disorder (MDD) and other mental disorders were depended on some subjective survey scales. There are confirmed relationship between the gut flora and the mental states of MDDpatients. METHODS: The V3-V4 region of the 16S rRNA gene was extracted from the fecal microbial communities in MDDpatients, PCR amplified and sequenced on the Illumina Miseq platform. RESULTS: More phylum Firmicutes, less Bacteroidetes, and more genus Prevotella, Klebsiella, Streptococcus and Clostridium XI were found in MDDpatients. The changes of the proportion of Prevotella and Klebsiella were consistent with Hamilton depression rating scale. LIMITATIONS: The conclusion was limited by small sample sizes and potential uncontrollable influence factors on fecal microbiota. DISCUSSION: Prevotella and Klebsiella proportion in fecal microbial communities should be concerned in the diagnosis and therapeutic monitoring of MDD in future.
Authors: Denitsa Simeonova; Drozdstoy Stoyanov; Jean-Claude Leunis; Andre F Carvalho; Marta Kubera; Marianna Murdjeva; Michael Maes Journal: Neurotox Res Date: 2019-12-04 Impact factor: 3.911
Authors: Narek Israelyan; Andrew Del Colle; Zhishan Li; Yeji Park; Albert Xing; Jacob P R Jacobsen; Ruth Ann Luna; Dane D Jensen; Moneek Madra; Virginia Saurman; Ray Rahim; Rocco Latorre; Kimberly Law; William Carson; Nigel W Bunnett; Marc G Caron; Kara G Margolis Journal: Gastroenterology Date: 2019-05-07 Impact factor: 22.682