Literature DB >> 27734398

TGF-β1- and CCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells.

Philip C Trackman1, Debashree Saxena2, Manish V Bais2.   

Abstract

A simple method for the determination of relative levels of insoluble collagen accumulation in fibroblast cultures is presented. Confluent cell cultures are provided with sodium ascorbate which is then permissive for collagen deposition. At intervals, cultures are fixed and stained successively with sirius red and then crystal Violet to, respectively, assess for relative changes in collagen accumulation in response to factors such as TGF-β1 or matricellular CCN2 and changes in DNA content as an index of changes in cell density.

Entities:  

Keywords:  Cell culture; Collagen accumulation; Collagen deposition; Fibroblast; Fibrosis; Sirius Red

Mesh：

Substances：

Year:  2017        PMID： 27734398     DOI： 10.1007/978-1-4939-6430-7_39

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

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Authors:  Ceylan Türlü; Nicholas Willumsen; Debora Marando; Peter Schjerling; Edyta Biskup; Jens Hannibal; Lars N Jorgensen; Magnus S Ågren
Journal:  Int J Mol Sci       Date:  2021-02-05       Impact factor: 5.923

2.  Low copper levels measured in the aortic wall of New Zealand patients with non-syndromic ascending thoracic aortic aneurysm.

Authors:  Adam El-Gamel; Josephenine Mak; Steve Bird; Megan N C Grainger; Gregory M Jacobson
Journal:  Interact Cardiovasc Thorac Surg       Date:  2022-09-09

3.  GED-0507 attenuates lung fibrosis by counteracting myofibroblast transdifferentiation in vivo and in vitro.

Authors:  Silvia Speca; Caroline Dubuquoy; Christel Rousseaux; Philippe Chavatte; Pierre Desreumaux; Paolo Spagnolo
Journal:  PLoS One       Date:  2021-09-16       Impact factor: 3.240
  3 in total

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