| Literature DB >> 27734386 |
Kei Sakamoto1, Yuki Matsushita2, Tokutaro Minamizato2, Yuko Katsuki2, Ken-Ichi Katsube2, Akira Yamaguchi2.
Abstract
Bone tissue is intrinsically hard and thus, it is more difficult to handle, process, and examine than soft tissues. Here, we describe an experimental model of bone regeneration and several selected protocols useful for investigating mRNA and protein expression in bone. The inhibitory function of CCN3 on membranous bone formation has been confirmed by following the protocols described herein (Fig. 1).Entities:
Keywords: Bone regeneration; Drill hole injury; HE staining; Immunohistochemistry; Primary osteoblastic cell culture
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Year: 2017 PMID： 27734386 DOI： 10.1007/978-1-4939-6430-7_27
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745