| Literature DB >> 27734368 |
Hiroshi Hanagata1, Makoto Mizukami2.
Abstract
Brevibacillus choshinensis is an excellent host for the production of secretory proteins. This host has also been applied successfully to efficient production of CCN proteins. Described herein are methods of constructing plasmids for CCN protein production (IGFBP-, VWC-, TSP-, and CT-domain) with Brevibacillus as a host, cultivation methods for protein production, and methods of purification for domain proteins using his-tag.Entities:
Keywords: BIC system; Brevibacillus choshinensis; IMAC
Mesh：
Substances：
Year: 2017 PMID： 27734368 DOI： 10.1007/978-1-4939-6430-7_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745