| Literature DB >> 2773316 |
G A De Zoeten1, J R Penswick, M A Horisberger, P Ahl, M Schultze, T Hohn.
Abstract
The ORF II of Cauliflower mosaic virus (CaMV) DNA was replaced with the human IFN alpha D coding sequence to yield a stable CaMV strain designated Ca524i. Inoculation of turnip (Brassica rapa cv "Just Right") with strain Ca524i DNA excised from plasmid pCa524i resulted in the production of biologically active IFN alpha D in infected plants. This was also true for its mutant (Ca562i) where one of the CYS codons was deleted. IFN alpha D produced in planta did not hamper superinfection with a single-stranded (+) sense RNA plant virus, turnip yellow mosaic virus (TYMV). Antibody gold labeling techniques and electron microscopy of infected plants showed that IFN was localized in the CaMV inclusion bodies.Entities:
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Year: 1989 PMID： 2773316 DOI： 10.1016/0042-6822(89)90123-2
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616