Natasha Pahuja1, Nimisha R Kumar2, Rushad Shroff1, Rohit Shetty1, Rudy M M A Nuijts3, Anuprita Ghosh2, Abhijit Sinha-Roy2, Shyam S Chaurasia4, Rajiv R Mohan5, Arkasubhra Ghosh6. 1. Cornea Department, Narayana Nethralaya, Bangalore, India. 2. GROW Research Laboratory, Narayana Nethralaya Foundation, Bangalore, India. 3. Cornea Clinic, Department of Ophthalmology, Maastricht University Medical Center, the Netherlands. 4. Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, Missouri, United States. 5. Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, Missouri, United States 5Mason Eye Institute, School of Medicine, University of Missouri, Columbia, Missouri, United States 6Harry S. Truman Veterans' Memorial Hospital, Columbia, Missouri, United States. 6. GROW Research Laboratory, Narayana Nethralaya Foundation, Bangalore, India 7Singapore Eye Research Institute, Singapore.
Abstract
PURPOSE: In this study, we elucidated the differential expression of a set of local molecular factors in ectatic cone area of the cornea to uncover a functional cause for focal corneal weakening characteristic of the keratoconus (KC) disease. METHODS: All human corneal samples were collected after approval of Institutional Ethics Committee and informed consent. Keratoconus patients were classified based on clinical parameters, topographical features, and structural deformity. Epithelial cells were collected from KC patients (n = 66) undergoing corneal cross-linking procedures from cone apex and periphery. Nonectatic refractive surgery patients (n = 23) served as controls. The ratio of epithelial gene expression in cone and periphery of each eye was estimated by quantitative PCR and correlated with clinical data. Similar cone versus periphery analysis was done from the KC stroma and from KC patients with Bowman's layer (BL) breach observed by anterior segment optical coherence tomography (OCT). RESULTS: Epithelium from the cone apex of KC patients had elevated levels of inflammatory factors TNF-α, IL-6, and matrix metalloproteinase 9 (MMP-9) but reduced Lysyl oxidase (LOX) and Collagen IVA1, which also demonstrated correlation with corneal curvature and deformity parameters. Stromal gene expression from KC patients showed trends similar to epithelium. Epithelium collected from the cone apex of BL breached KC patients showed significantly elevated MMP-9, TNF-α, and IL-6 levels but reduced IL-10, tissue inhibitor of metalloproteinases 1 (TIMP-1), and Collagen IVA1 expression. CONCLUSIONS: This study provides the first evidence that altered corneal epithelial and stromal expression of specific genes at the corneal cone apex drives focal structural weakness in KC.
PURPOSE: In this study, we elucidated the differential expression of a set of local molecular factors in ectatic cone area of the cornea to uncover a functional cause for focal corneal weakening characteristic of the keratoconus (KC) disease. METHODS: All human corneal samples were collected after approval of Institutional Ethics Committee and informed consent. Keratoconus patients were classified based on clinical parameters, topographical features, and structural deformity. Epithelial cells were collected from KC patients (n = 66) undergoing corneal cross-linking procedures from cone apex and periphery. Nonectatic refractive surgery patients (n = 23) served as controls. The ratio of epithelial gene expression in cone and periphery of each eye was estimated by quantitative PCR and correlated with clinical data. Similar cone versus periphery analysis was done from the KC stroma and from KC patients with Bowman's layer (BL) breach observed by anterior segment optical coherence tomography (OCT). RESULTS: Epithelium from the cone apex of KC patients had elevated levels of inflammatory factors TNF-α, IL-6, and matrix metalloproteinase 9 (MMP-9) but reduced Lysyl oxidase (LOX) and Collagen IVA1, which also demonstrated correlation with corneal curvature and deformity parameters. Stromal gene expression from KC patients showed trends similar to epithelium. Epithelium collected from the cone apex of BL breached KC patients showed significantly elevated MMP-9, TNF-α, and IL-6 levels but reduced IL-10, tissue inhibitor of metalloproteinases 1 (TIMP-1), and Collagen IVA1 expression. CONCLUSIONS: This study provides the first evidence that altered corneal epithelial and stromal expression of specific genes at the corneal cone apex drives focal structural weakness in KC.
Authors: Louisa M Bulirsch; Constance Weber; Marlene Saßmannshausen; Markus Kohlhaas; Frank G Holz; Karin U Loeffler; Martina C Herwig-Carl Journal: Ophthalmologe Date: 2021-12-07 Impact factor: 1.059