| Literature DB >> 27730616 |
Hanna Klang Årstrand1, Maria V Turkina2.
Abstract
This chapter describes a method of plant cytosolic ribosomes isolation typically used for further proteomic studies. Detailed description procedures including plant material disruption, various centrifugation steps, sucrose cushion centrifugation, and quality control of preparation are provided.Entities:
Keywords: Centrifugation; Cytosol; Ribosomal proteins; Ribosome; Sucrose cushion; Ultracentrifugation
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Year: 2017 PMID： 27730616 DOI： 10.1007/978-1-4939-6533-5_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745