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Literature DB >> 27730575

The Q-System: A Versatile Expression System for Drosophila.

Olena Riabinina¹, Christopher J Potter².

Abstract

Binary expression systems are flexible and versatile genetic tools in Drosophila. The Q-system is a recently developed repressible binary expression system that offers new possibilities for transgene expression and genetic manipulations. In this review chapter, we focus on current state-of-the-art Q-system tools and reagents. We also discuss in vivo applications of the Q-system, together with GAL4/UAS and LexA/LexAop systems, for simultaneous expression of multiple effectors, intersectional labeling, and clonal analysis.

Entities: CellLine Chemical Disease Gene Mutation Species

Keywords: Binary expression system; Chimeric transactivators; GAL4QF; Intersectional expression; LexAQF; MARCM; Mitotic recombination; Mosaic analysis; Neurospora crassa; Q-system; QF; QF2; QF2w; QS; QUAS; Quinic acid

Mesh：

Substances：

Year: 2016 PMID： 27730575 PMCID： PMC5270762 DOI： 10.1007/978-1-4939-6371-3_3

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

77 in total

Review 1. GAL4 system in Drosophila: a fly geneticist's Swiss army knife.

Authors: Joseph B Duffy
Journal: Genesis Date: 2002 Sep-Oct Impact factor: 2.487

2. Hexameric GFP and mCherry reporters for the Drosophila GAL4, Q, and LexA transcription systems.

Authors: Harold K Shearin; Ian S Macdonald; Laura P Spector; R Steven Stowers
Journal: Genetics Date: 2014-01-21 Impact factor: 4.562

Review 3. Genetic odyssey to generate marked clones in Drosophila mosaics.

Authors: Ruth Griffin; Richard Binari; Norbert Perrimon
Journal: Proc Natl Acad Sci U S A Date: 2014-03-12 Impact factor: 11.205

4. Antibody-promoted dimerization bypasses the regulation of DNA binding by the heme domain of the yeast transcriptional activator HAP1.

Authors: L Zhang; O Bermingham-McDonogh; B Turcotte; L Guarente
Journal: Proc Natl Acad Sci U S A Date: 1993-04-01 Impact factor: 11.205

5. Structure of the DNA-binding domain of zinc GAL4.

Authors: P J Kraulis; A R Raine; P L Gadhavi; E D Laue
Journal: Nature Date: 1992-04-02 Impact factor: 49.962

6. A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body.

Authors: Jena L Pitman; Wolf Huetteroth; Christopher J Burke; Michael J Krashes; Sen-Lin Lai; Tzumin Lee; Scott Waddell
Journal: Curr Biol Date: 2011-04-28 Impact factor: 10.834

7. Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects.

Authors: S T Sweeney; K Broadie; J Keane; H Niemann; C J O'Kane
Journal: Neuron Date: 1995-02 Impact factor: 17.173

8. Expansion of the gateway multisite recombination cloning toolkit.

Authors: Harold K Shearin; Alisa R Dvarishkis; Craig D Kozeluh; R Steven Stowers
Journal: PLoS One Date: 2013-10-18 Impact factor: 3.240

9. Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes.

Authors: Michele Markstein; Chrysoula Pitsouli; Christians Villalta; Susan E Celniker; Norbert Perrimon
Journal: Nat Genet Date: 2008-03-02 Impact factor: 38.330

10. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.

Authors: A H Brand; N Perrimon
Journal: Development Date: 1993-06 Impact factor: 6.868

14 in total

1. Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster.

Authors: Kirby M Donnelly; Margaret M P Pearce
Journal: J Vis Exp Date: 2018-03-12 Impact factor: 1.355

2. Phagocytic glia are obligatory intermediates in transmission of mutant huntingtin aggregates across neuronal synapses.

Authors: Kirby M Donnelly; Olivia R DeLorenzo; Aprem DA Zaya; Gabrielle E Pisano; Wint M Thu; Liqun Luo; Ron R Kopito; Margaret M Panning Pearce
Journal: Elife Date: 2020-05-28 Impact factor: 8.140

The Q-System: A Versatile Expression System for Drosophila.

Review 1. GAL4 system in Drosophila: a fly geneticist's Swiss army knife.

2. Hexameric GFP and mCherry reporters for the Drosophila GAL4, Q, and LexA transcription systems.

Review 3. Genetic odyssey to generate marked clones in Drosophila mosaics.

4. Antibody-promoted dimerization bypasses the regulation of DNA binding by the heme domain of the yeast transcriptional activator HAP1.

5. Structure of the DNA-binding domain of zinc GAL4.

6. A pair of inhibitory neurons are required to sustain labile memory in the Drosophila mushroom body.

7. Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects.

8. Expansion of the gateway multisite recombination cloning toolkit.

9. Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes.

10. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.

1. Monitoring Cell-to-cell Transmission of Prion-like Protein Aggregates in Drosophila Melanogaster.

2. Phagocytic glia are obligatory intermediates in transmission of mutant huntingtin aggregates across neuronal synapses.

3. Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange.

4. Characterizing a gene expression toolkit for eye- and photoreceptor-specific expression in Drosophila.

5. Split-QF System for Fine-Tuned Transgene Expression in Drosophila.

6. Synthetic control devices for gene regulation in Penicillium chrysogenum.

7. Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator.

Review 8. Functional Imaging and Optogenetics in Drosophila.

9. Non-apoptotic caspase activation preserves Drosophila intestinal progenitor cells in quiescence.

10. Functional Interaction Between Drosophila Olfactory Sensory Neurons and Their Support Cells.