Wang-Dong Xu1, Lin-Chong Su1, Qi-Bing Xie1, Yi Zhao1, Yi Liu2. 1. Department of Rheumatology and Immunology, West China Hospital, Sichuan University, 37 Guoxue Xiang, Chengdu, Sichuan 610041, PR China. 2. Department of Rheumatology and Immunology, West China Hospital, Sichuan University, 37 Guoxue Xiang, Chengdu, Sichuan 610041, PR China. Electronic address: yi2006liu@163.com.
Abstract
BACKGROUND: Interleukin-2 inducible T-cell kinase (ITK) is expressed in T cells, and plays an important role in autoimmune inflammatory diseases through regulating the balance of Th17/Treg. However, its role in human systemic lupus erythematosus (SLE) remains unclear. The present study aims to measure the activation status of ITK in T cells from SLE patients and healthy controls, and identify its possible correlation to disease severity. We also discuss the serum levels of Th17, Treg related cytokines including IL-17, IL-21, IL-22, IL-10, analyzing correlation between ITK and Th17/Treg related cytokines. METHODS: Peripheral blood samples were drawn from 42 patients with SLE and 43 healthy blood donors, and the phosphorylation of ITK protein was studied in T cells using flow cytometry. In addition, serum levels of Th17/Treg related cytokines were studied with enzyme-linked immunosorbent assay (ELISA). RESULTS: Percentages of CD4+pITK+ T cells, CD8+pITK+ T cells were higher in SLE patients compared with controls, and were positively related to disease activity, some clinical and laboratory parameters. Percentages of CD4+pITK+ T cells, CD8+pITK+ T cells were more prominent in active SLE patients compared with less active patients. Serum levels of Th17 and Treg related cytokines were higher in patients compared with controls. CD4+pITK+ T cells were related to levels of IL-17, IL-21. CONCLUSION: These data indicate that increased ITK expression could act as a disease activity marker and as a risk factor for involvement in SLE, but it still needs further study to confirm. Copyright Â
BACKGROUND:Interleukin-2 inducible T-cell kinase (ITK) is expressed in T cells, and plays an important role in autoimmune inflammatory diseases through regulating the balance of Th17/Treg. However, its role in humansystemic lupus erythematosus (SLE) remains unclear. The present study aims to measure the activation status of ITK in T cells from SLEpatients and healthy controls, and identify its possible correlation to disease severity. We also discuss the serum levels of Th17, Treg related cytokines including IL-17, IL-21, IL-22, IL-10, analyzing correlation between ITK and Th17/Treg related cytokines. METHODS: Peripheral blood samples were drawn from 42 patients with SLE and 43 healthy blood donors, and the phosphorylation of ITK protein was studied in T cells using flow cytometry. In addition, serum levels of Th17/Treg related cytokines were studied with enzyme-linked immunosorbent assay (ELISA). RESULTS: Percentages of CD4+pITK+ T cells, CD8+pITK+ T cells were higher in SLEpatients compared with controls, and were positively related to disease activity, some clinical and laboratory parameters. Percentages of CD4+pITK+ T cells, CD8+pITK+ T cells were more prominent in active SLEpatients compared with less active patients. Serum levels of Th17 and Treg related cytokines were higher in patients compared with controls. CD4+pITK+ T cells were related to levels of IL-17, IL-21. CONCLUSION: These data indicate that increased ITK expression could act as a disease activity marker and as a risk factor for involvement in SLE, but it still needs further study to confirm. Copyright Â
Authors: Kodappully S Siveen; Kirti S Prabhu; Iman W Achkar; Shilpa Kuttikrishnan; Sunitha Shyam; Abdul Q Khan; Maysaloun Merhi; Said Dermime; Shahab Uddin Journal: Mol Cancer Date: 2018-02-19 Impact factor: 27.401