Stefania Boccia1, Ida Torre2, Lidia Santarpia3, Carmela Iervolino2, Concetta Del Piano4, Anna Puggina1, Roberta Pastorino5, Miroslav Dragic5, Rosarita Amore5, Tonia Borriello2, Raffaele Palladino2, Francesca Pennino2, Franco Contaldo4, Fabrizio Pasanisi4. 1. Section of Hygiene - Institute of Public Health, Università Cattolica del Sacro Cuore, Fondazione Policlinico Universitario "Agostino Gemelli", L.go F. Vito, 1, 00168 Rome, Italy. 2. Department of Public Health, University of Naples "Federico II", Naples, Italy. 3. Internal Medicine and Clinical Nutrition Unit, Department of Clinical Medicine and Surgery, Federico II University, Naples, Italy. Electronic address: lidia.santarpia@unina.it. 4. Internal Medicine and Clinical Nutrition Unit, Department of Clinical Medicine and Surgery, Federico II University, Naples, Italy. 5. Section of Hygiene - Institute of Public Health, Università Cattolica del Sacro Cuore, L.go F. Vito, 1, 00168 Rome, Italy.
Abstract
INTRODUCTION: Intestinal bacterial flora plays a central role in human intestinal health and disease. Short Bowel Syndrome (SBS), a clinical condition deriving from extensive bowel resections, influence intestinal microbiota (IM) composition in order to reach a new metabolic balance. Little is known about IM in adult patients after wide intestinal resections. MATERIAL AND METHODS: Fecal samples from 12 SBS patients and 16 controls were analyzed in their microbial profile by using both culture-dependent method and quantitative Real-Time PCR (qRT-PCR). RESULTS: The two methods revealed significant lower concentrations of Bacteroidetes (p-value = .02), Firmicutes (p-value = .05), Bifidobacterium (p-value < .01), and Methanobrevibacter Smithii (p-value = .04) in SBS patients than controls. CONCLUSIONS: The significantly different fecal microbiome in SBS patients compared with healthy controls could open new perspectives on the care of their intestinal functions.
INTRODUCTION: Intestinal bacterial flora plays a central role in human intestinal health and disease. Short Bowel Syndrome (SBS), a clinical condition deriving from extensive bowel resections, influence intestinal microbiota (IM) composition in order to reach a new metabolic balance. Little is known about IM in adult patients after wide intestinal resections. MATERIAL AND METHODS: Fecal samples from 12 SBSpatients and 16 controls were analyzed in their microbial profile by using both culture-dependent method and quantitative Real-Time PCR (qRT-PCR). RESULTS: The two methods revealed significant lower concentrations of Bacteroidetes (p-value = .02), Firmicutes (p-value = .05), Bifidobacterium (p-value < .01), and Methanobrevibacter Smithii (p-value = .04) in SBSpatients than controls. CONCLUSIONS: The significantly different fecal microbiome in SBSpatients compared with healthy controls could open new perspectives on the care of their intestinal functions.