Effects of Leaf Removal and Applied Water on Flavonoid Accumulation in Grapevine (Vitis vinifera L. cv. Merlot) Berry in a Hot Climate.

The relationships between variations in grapevine (Vitis vinifera L. cv. Merlot) fruit zone light exposure and water deficits and the resulting berry flavonoid composition were investigated in a hot climate. The experimental design involved application of mechanical leaf removal (control, pre-bloom, post-fruit set) and differing water deficits (sustained deficit irrigation and regulated deficit irrigation). Flavonol and anthocyanin concentrations were measured by C18 reversed-phased HPLC and increased with pre-bloom leaf removal in 2013, but with post-fruit set leaf removal in 2014. Proanthocyanidin isolates were characterized by acid catalysis in the presence of excess phloroglucinol followed by reversed-phase HPLC. Post-fruit set leaf removal increased total proanthocyanidin concentration in both years, whereas no effect was observed with applied water amounts. Mean degree of polymerization of skin proanthocyanidins increased with post-fruit set leaf removal compared to pre-bloom, whereas water deficit had no effect. Conversion yield was greater with post-fruit set leaf removal. Seed proanthocyanidin concentration was rarely affected by applied treatments. The application of post-fruit set leaf removal, regardless of water deficit. increased the proportion of proanthocyanidins derived from the skin, whereas no leaf removal or pre-bloom leaf removal regardless of water deficit increased the proportion of seed-derived proanthocyanidins. The study provides fundamental information to viticulturists and winemakers on how to manage red wine grape low molecular weight phenolics and polymeric proanthocyanidin composition in a hot climate.