Literature DB >> 27723090

Flow Analysis and Sorting of Plant Chromosomes.

Jan Vrána1, Petr Cápal1, Hana Šimková1, Miroslava Karafiátová1, Jana Čížková1, Jaroslav Doležel1.   

Abstract

Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed. © 2016 by John Wiley & Sons, Inc.
Copyright © 2016 John Wiley & Sons, Inc.

Entities:  

Keywords:  cell cycle synchronization; chromosome genomics; chromosome isolation; chromosome sorting; chromosome-specific DNA libraries; flow cytometry; genome sequencing; high-molecular-weight DNA; physical genome mapping; plant chromosomes

Mesh：

Substances：

Year:  2016        PMID： 27723090     DOI： 10.1002/cpcy.9

Source DB:  PubMed          Journal:  Curr Protoc Cytom        ISSN： 1934-9297


  8 in total

1.  Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa.

Authors:  Wojciech Jesionek; Markéta Bodláková; Zdeněk Kubát; Radim Čegan; Boris Vyskot; Jan Vrána; Jan Šafář; Janka Puterova; Roman Hobza
Journal:  Ann Bot       Date:  2021-01-01       Impact factor: 4.357

2.  Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids.

Authors:  Edina Türkösi; László Ivanizs; András Farkas; Eszter Gaál; Klaudia Kruppa; Péter Kovács; Éva Szakács; Kitti Szőke-Pázsi; Mahmoud Said; Petr Cápal; Simon Griffiths; Jaroslav Doležel; István Molnár
Journal:  Front Plant Sci       Date:  2022-06-13       Impact factor: 6.627

3.  Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.

Authors:  Yan Li; Aymeric Leveau; Qiang Zhao; Qi Feng; Hengyun Lu; Jiashun Miao; Zheyong Xue; Azahara C Martin; Eva Wegel; Jing Wang; Anastasia Orme; Maria-Dolores Rey; Miroslava Karafiátová; Jan Vrána; Burkhard Steuernagel; Ryan Joynson; Charlotte Owen; James Reed; Thomas Louveau; Michael J Stephenson; Lei Zhang; Xuehui Huang; Tao Huang; Danling Fan; Congcong Zhou; Qilin Tian; Wenjun Li; Yiqi Lu; Jiaying Chen; Yan Zhao; Ying Lu; Chuanrang Zhu; Zhenhua Liu; Guy Polturak; Rebecca Casson; Lionel Hill; Graham Moore; Rachel Melton; Neil Hall; Brande B H Wulff; Jaroslav Doležel; Tim Langdon; Bin Han; Anne Osbourn
Journal:  Nat Commun       Date:  2021-05-07       Impact factor: 17.694

4.  Identification of a Dominant Chlorosis Phenotype Through a Forward Screen of the Triticum turgidum cv. Kronos TILLING Population.

Authors:  Sophie A Harrington; Nicolas Cobo; Miroslava Karafiátová; Jaroslav Doležel; Philippa Borrill; Cristobal Uauy
Journal:  Front Plant Sci       Date:  2019-07-24       Impact factor: 5.753

5.  Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars.

Authors:  Cushla J Metcalfe; Jingchuan Li; Debora Giorgi; Jaroslav Doležel; Nathalie Piperidis; Karen S Aitken
Journal:  Sci Rep       Date:  2019-12-18       Impact factor: 4.379

6.  Flow karyotyping of wheat-Aegilops additions facilitate dissecting the genomes of Ae. biuncialis and Ae. geniculata into individual chromosomes.

Authors:  Mahmoud Said; Petr Cápal; András Farkas; Eszter Gaál; László Ivanizs; Bernd Friebe; Jaroslav Doležel; István Molnár
Journal:  Front Plant Sci       Date:  2022-10-03       Impact factor: 6.627

7.  Integration of Genetic and Cytogenetic Maps and Identification of Sex Chromosome in Garden Asparagus (Asparagus officinalis L.).

Authors:  Roberto Moreno; Patricia Castro; Jan Vrána; Marie Kubaláková; Petr Cápal; Verónica García; Juan Gil; Teresa Millán; Jaroslav Doležel
Journal:  Front Plant Sci       Date:  2018-07-31       Impact factor: 5.753

Review 8.  Chromosome analysis and sorting.

Authors:  Jaroslav Doležel; Sergio Lucretti; István Molnár; Petr Cápal; Debora Giorgi
Journal:  Cytometry A       Date:  2021-02-21       Impact factor: 4.355
  8 in total

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