Whole-genome sequence of Dermabacter vaginalis AD1-86T, isolated from vaginal fluid of Korean woman.

Dermabacter vaginalis AD1-86T was isolated from the vaginal fluid of a Korean woman. Whole genome sequencing analysis was conducted using a PacBio RS II platform and annotated on RAST. The nucleotide sequence of this genome was deposited into DDBJ/EMBL/GenBank under the accession NZ_CP012117.

Direct link to deposited data

http://www.ncbi.nlm.nih.gov/nuccore/NZ_CP012117

Experimental design, materials and methods

Strains of the genus Dermabacter were first isolated from human skin and have subsequently been isolated from a variety of clinical specimens, such as blood cultures, fatal septicaemia, cutaneous abscesses, peritoneal fluid, a brain biopsy specimen and chronic osteomyelitis, suggesting this microorganism to be an opportunistic pathogen [1]. Recently, a novel species of the genus Dermabter were isolated for the first time from the human vaginal fluid and assigned to a novel genus and species, Dermabacter vaginalis AD1-86T (= KCTC 39585T = DSM 100050T) [1]. Genomic DNA of strain AD1-86T was extracted using bead-beating technique [2] and sequenced on PacBio RS II instrument (Pacific Biosciences). De novo genome assembly was performed using CLCbio CLC Genomics Workbench v7.5 and annotation was conducted on RAST [3]. SEED viewer was used for subsystem functional categorization of the predicted open reading frames (ORFs) and for visualization [4].

Data description

The genome of D. vaginalis AD1-86T consisted of single circular DNA chromosome of 2,392,314 base pairs, a GC content of 62.6%. The genome was predicted to contain 2088 coding sequence, 48 tRNAs and 10 rRNAs. These genes were annotated and classified into 308 subsystems (Fig. 1). Most of the annotated genes were involved in protein metabolism (209), carbohydrate metabolism (187), amino acids and derivatives (175), cofactors, vitamins, prosthetic groups and pigments formations (150) (Fig. 1).

Fig. 1

Subsystems of Dermabacter vaginalis AD1-86YT based on SEED database.

Nucleotide accession number

This whole genome project has been deposited at DDBJ/EMBL/GenBank under accession no. NZ_CP012117.

Conflict of interest

The authors clarified that this work and writing has no conflict of interest.

Specifications
Organism/cell line/tissue	Dermabacter vaginalis
Strain	AD1-86T
Sequencer or array type	PacBio RS II
Data format	Analyzed
Experimental factors	Microbial strain
Experimental features	Whole genome analysis and gene annotation of AD1-86 T
Consent	N/A
Sample source location	Vagina