Gui Yong-Xian1, Li Xiao-Huan2, Zhang Fan3, Tian Guo-Fang1. 1. Department of Oncology, Xinxiang Central Hospital of Henan Province, Henan 453000, PR China. 2. Department of Endoscopy, Xinxiang Central Hospital of Henan Province, Henan 453000, PR China. 3. Department of Oncology, 1st Affiliated Hospital of Fujian Medical University, Fuzhou 350004, PR China.
Abstract
AIM: The aim of the study is to investigate the underlying molecular mechanisms by which gemcitabine (gem) inhibits proliferation and induces apoptosis in human pancreatic cancer PANC-1 cells in vitro. MATERIALS AND METHODS: After PANC-1 cells had been treated by indicated concentration (0, 5, and 25 mg/L) of gem for 48 h, cell proliferation was evaluated by 3'-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay; cell morphology was observed by transmission electron microscopy; Expression of c-IAP2 and Bcl-2 proteins was analyzed by Western blot; the activity of caspase-3 and -9 was detected by spectrophotometry. RESULTS: Gem significantly inhibited cell proliferation and could induce apoptosis of human pancreatic cancer PANC-1 cells, with a dose-dependent manner. Western blot analysis showed that gem significantly reduced c-IAP2 and Bcl-2 proteins expression level (P < 0.05). Spectrophotometric assay showed that gem significantly increased caspase-3 and -9 activity in PANC-1 cells. CONCLUSION: Gem could induce apoptosis of human pancreatic cancer PANC-1 cells, probably through downregulating c-IAP2 and Bcl-2 expression levels, and at the same time activating caspase-3 and -9.
AIM: The aim of the study is to investigate the underlying molecular mechanisms by which gemcitabine (gem) inhibits proliferation and induces apoptosis in humanpancreatic cancer PANC-1 cells in vitro. MATERIALS AND METHODS: After PANC-1 cells had been treated by indicated concentration (0, 5, and 25 mg/L) of gem for 48 h, cell proliferation was evaluated by 3'-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay; cell morphology was observed by transmission electron microscopy; Expression of c-IAP2 and Bcl-2 proteins was analyzed by Western blot; the activity of caspase-3 and -9 was detected by spectrophotometry. RESULTS:Gem significantly inhibited cell proliferation and could induce apoptosis of humanpancreatic cancer PANC-1 cells, with a dose-dependent manner. Western blot analysis showed that gem significantly reduced c-IAP2 and Bcl-2 proteins expression level (P < 0.05). Spectrophotometric assay showed that gem significantly increased caspase-3 and -9 activity in PANC-1 cells. CONCLUSION:Gem could induce apoptosis of humanpancreatic cancer PANC-1 cells, probably through downregulating c-IAP2 and Bcl-2 expression levels, and at the same time activating caspase-3 and -9.
Authors: Alexandria Turner; Danielle R Bond; Quan V Vuong; Anita Chalmers; Emma L Beckett; Judith Weidenhofer; Christopher J Scarlett Journal: Mol Biol Rep Date: 2020-02-17 Impact factor: 2.316