Influence of non-starchy polysaccharides on barley milling behavior and evaluating bioactive composition of milled fractions.

Hulless barley cultivars grown at various altitudes were subjected to different conditioning treatments prior to roller milling. Amongst all treatments, conditioning grains to a moisture content of 14% for 30min was found to be optimum. The bran fractions displayed greater levels of non-starchy polysaccharides and bioactive components as compared to refined flour fraction. The presence of greater levels of β-glucan in whole barley flour and bran of high altitude cultivars affected the refined flour yield inversely. Cultivars having higher total and insoluble arabinoxylans also resulted in lower flour yields (R=-0.76; R=-0.73). The damaged starch content of barley cultivars ranged between 5.1% and 8.7% which correlated positively with the content of β-glucans (up to R=0.77) and arabinoxylans (up to R=0.80) in bran and refined flour fractions. The anthocyanin and total phenolic contents of refined flours ranged between 3.9-7.6μg/g and 1299-1607μg FAE/g and was higher for high altitude cultivars.