| Literature DB >> 27718201 |
Alicia M Schwartz1, Michelle Y Chan1, Dawn M Fedor1, Sandra J Sivananthan1, Ryan M Kramer2.
Abstract
Adjuvants in modern vaccines boost and shape immune responses and allow for antigen dose-sparing. Analysis of protein antigens in the presence of adjuvants can prove challenging, especially if the adjuvant interferes with visualization of the protein band on an SDS-PAGE gel. In this chapter, a variety of different techniques are presented to mitigate the interference of a nanoemulsion adjuvant, GLA-SE, with different recombinant proteins of varying molecular weight by addressing sample preparation and staining methods.Entities:
Keywords: Adjuvant; Antigen; Coomassie stain; Gold stain; PVDF membrane; SDS-PAGE; Silver stain
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Year: 2017 PMID： 27718201 DOI： 10.1007/978-1-4939-6445-1_20
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745