| Literature DB >> 27718094 |
Yue Zhang1,2,3, Chun-Yan Lin1,2,3, Xiao-Mei Li1,2,3, Zheng-Kun Tang1,2,3, Jianjun Qiao1,2,3, Guang-Rong Zhao4,5,6.
Abstract
The polyether ionophore antibiotic monensin is produced by Streptomyces cinnamonensis and is used as a coccidiostat for chickens and growth-promoting agent for cattle. Monensin biosynthetic gene cluster has been cloned and partially characterized. The GntR-family transcription factor DasR regulates antibiotic production and morphological development in Streptomyces coelicolor and Saccharopolyspora erythraea. In this study, we identified and characterized the two-level regulatory cascade of DasR to monensin production in S. cinnamonensis. Forward and reverse genetics by overexpression and antisense RNA silence of dasR revealed that DasR positively controls monensin production under nutrient-rich condition. Electrophoresis mobility shift assay (EMSA) showed that DasR protein specifically binds to the promoter regions of both pathway-specific regulatory gene monRII and biosynthetic genes monAIX, monE and monT. Semi-quantitative RT-PCR further confirmed that DasR upregulates the transcriptional levels of these genes during monensin fermentation. Subsequently, co-overexpressed dasR with pathway-specific regulatory genes monRI, monRII or monH greatly improved monensin production.Entities:
Keywords: Monensin dasR; Secondary metabolism; Streptomyces cinnamonensis; Transcription factor
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Year: 2016 PMID： 27718094 DOI： 10.1007/s10295-016-1845-4
Source DB: PubMed Journal: J Ind Microbiol Biotechnol ISSN： 1367-5435 Impact factor: 3.346