PURPOSE: To optimize the production of hyperpolarized 13 C-bicarbonate from the decarboxylation of hyperpolarized [1-13 C]pyruvate and use it to image pH in the lungs and heart of rats with acute lung injury. METHODS: Two forms of catalysis are compared calorimetrically to maximize the rate of decarboxylation and rapidly produce hyperpolarized bicarbonate from pyruvate while minimizing signal loss. Rats are injured using an acute lung injury model combining ventilator-induced lung injury and acid aspiration. Carbon images are obtained from both healthy (n = 4) and injured (n = 4) rats using a slice-selective chemical shift imaging sequence with low flip angle. pH is calculated from the relative HCO3- and CO2 signals using the Henderson-Hasselbalch equation. RESULTS: It is demonstrated that base catalysis is more effective than metal-ion catalysis for this decarboxylation reaction. Bicarbonate polarizations up to 17.2% are achieved using the base-catalyzed reaction. A mean pH difference between lung and heart of 0.14 pH units is measured in the acute lung injury model. A significant pH difference between injured and uninjured lungs is also observed. CONCLUSION: It is demonstrated that hyperpolarized 13 C-bicarbonate can be efficiently produced from the base-catalyzed decarboxylation of pyruvate. This method is used to obtain the first regional pH image of the lungs and heart of an animal. Magn Reson Med 78:1121-1130, 2017.
PURPOSE: To optimize the production of hyperpolarized 13 C-bicarbonate from the decarboxylation of hyperpolarized [1-13 C]pyruvate and use it to image pH in the lungs and heart of rats with acute lung injury. METHODS: Two forms of catalysis are compared calorimetrically to maximize the rate of decarboxylation and rapidly produce hyperpolarized bicarbonate from pyruvate while minimizing signal loss. Rats are injured using an acute lung injury model combining ventilator-induced lung injury and acid aspiration. Carbon images are obtained from both healthy (n = 4) and injured (n = 4) rats using a slice-selective chemical shift imaging sequence with low flip angle. pH is calculated from the relative HCO3- and CO2 signals using the Henderson-Hasselbalch equation. RESULTS: It is demonstrated that base catalysis is more effective than metal-ion catalysis for this decarboxylation reaction. Bicarbonate polarizations up to 17.2% are achieved using the base-catalyzed reaction. A mean pH difference between lung and heart of 0.14 pH units is measured in the acute lung injury model. A significant pH difference between injured and uninjured lungs is also observed. CONCLUSION: It is demonstrated that hyperpolarized 13 C-bicarbonate can be efficiently produced from the base-catalyzed decarboxylation of pyruvate. This method is used to obtain the first regional pH image of the lungs and heart of an animal. Magn Reson Med 78:1121-1130, 2017.
Authors: J Vaughan; L Ngamtrakulpanit; T N Pajewski; R Turner; T A Nguyen; A Smith; P Urban; S Hom; B Gaston; J Hunt Journal: Eur Respir J Date: 2003-12 Impact factor: 16.671
Authors: Robert Matthew Kottmann; Ajit A Kulkarni; Katie A Smolnycki; Elizabeth Lyda; Thinesh Dahanayake; Rami Salibi; Sylvie Honnons; Carolyn Jones; Nancy G Isern; Jian Z Hu; Steven D Nathan; Geraldine Grant; Richard P Phipps; Patricia J Sime Journal: Am J Respir Crit Care Med Date: 2012-08-23 Impact factor: 21.405
Authors: Ferdia A Gallagher; Mikko I Kettunen; Sam E Day; De-En Hu; Jan Henrik Ardenkjaer-Larsen; René in 't Zandt; Pernille R Jensen; Magnus Karlsson; Klaes Golman; Mathilde H Lerche; Kevin M Brindle Journal: Nature Date: 2008-05-28 Impact factor: 49.962
Authors: Mehrdad Pourfathi; Yi Xin; Stephen J Kadlecek; Maurizio F Cereda; Harrilla Profka; Hooman Hamedani; Sarmad M Siddiqui; Kai Ruppert; Nicholas A Drachman; Jennia N Rajaei; Rahim R Rizi Journal: Magn Reson Med Date: 2017-01-11 Impact factor: 4.668
Authors: David E Korenchan; Jeremy W Gordon; Sukumar Subramaniam; Renuka Sriram; Celine Baligand; Mark VanCriekinge; Robert Bok; Daniel B Vigneron; David M Wilson; Peder E Z Larson; John Kurhanewicz; Robert R Flavell Journal: Magn Reson Med Date: 2019-05-03 Impact factor: 4.668
Authors: John Kurhanewicz; Daniel B Vigneron; Jan Henrik Ardenkjaer-Larsen; James A Bankson; Kevin Brindle; Charles H Cunningham; Ferdia A Gallagher; Kayvan R Keshari; Andreas Kjaer; Christoffer Laustsen; David A Mankoff; Matthew E Merritt; Sarah J Nelson; John M Pauly; Philips Lee; Sabrina Ronen; Damian J Tyler; Sunder S Rajan; Daniel M Spielman; Lawrence Wald; Xiaoliang Zhang; Craig R Malloy; Rahim Rizi Journal: Neoplasia Date: 2018-11-23 Impact factor: 5.715
Authors: Usman Arshad; Henry Pertinez; Helen Box; Lee Tatham; Rajith K R Rajoli; Paul Curley; Megan Neary; Joanne Sharp; Neill J Liptrott; Anthony Valentijn; Christopher David; Steve P Rannard; Paul M O'Neill; Ghaith Aljayyoussi; Shaun H Pennington; Stephen A Ward; Andrew Hill; David J Back; Saye H Khoo; Patrick G Bray; Giancarlo A Biagini; Andrew Owen Journal: Clin Pharmacol Ther Date: 2020-06-14 Impact factor: 6.903