Aleksander J Owczarek1, Magdalena Olszanecka-Glinianowicz2, Piotr Kocełak2, Maria Bożentowicz-Wikarek3, Aniceta Brzozowska2, Małgorzata Mossakowska4, Monika Puzianowska-Kuźnicka5,6, Tomasz Grodzicki7, Andrzej Więcek8, Jerzy Chudek3. 1. a Department of Statistics, Department of Instrumental Analysis, Faculty of Pharmacy and Laboratory Medicine in Sosnowiec , Medical University of Silesia , Katowice , Poland. 2. b Health Promotion and Obesity Management Unit, Department of Pathophysiology , Medical Faculty in Katowice, Medical University of Silesia , Katowice , Poland. 3. c Pathophysiology Unit, Department of Pathophysiology , Medical Faculty in Katowice, Medical University of Silesia , Katowice , Poland. 4. d International Institute of Molecular and Cell Biology , Warsaw , Poland. 5. e Department of Human Epigenetics , Mossakowski Medical Research Centre , Warsaw , Poland. 6. f Department of Geriatrics and Gerontology , Medical Center of Postgraduate Education , Warsaw , Poland. 7. g Department of Internal Medicine and Gerontology , Jagiellonian University Medical College , Krakow , Poland. 8. h Department of Nephrology, Transplantation and Internal Medicine, Medical Faculty in Katowice , Medical University of Silesia , Katowice , Poland.
Abstract
BACKGROUND: The available literature suggests that circulating visfatin/Nicotinamide Phosphoribosyltransferase (NAMPT) level variability in humans is related to obesity, insulin resistance, inflammation, and lipid profile. The aim of the study was to assess the relationship between circulating visfatin/NAMPT, obesity, insulin resistance, inflammation, and lipid profile in a large population-based, elderly cohort, applying structural equation modeling. MATERIALS AND METHODS: The analysis included 2983 elderly participants of the PolSenior study with assessed total blood count, fasting concentrations of lipids, glucose, insulin, hs-CRP, interleukin-6, and visfatin/NAMPT (by ELISA), and calculated HOMA-IR. RESULTS: The circulating visfatin/NAMPT levels were higher in obese compared to normal weight subjects, in those with hs-CRP above 3 mg/L, with low serum HDL cholesterol, and in insulin resistant subjects. Based on results of the exploratory factor analysis, a baseline model of mutual relationship between four latent and measured variables was created and a final model was developed by maintaining only two significant categories. The important variables for 'latent inflammation' proved to be hs-CRP and IL-6 serum levels. In the case of 'nutritional status', important variables were BMI, waist circumference, and to a lesser extent insulin resistance. Additionally, the residual correlation between those two constructs was also statistically significant. CONCLUSION: The structural equation modeling provided support for the existence of a link between nutritional status, inflammation and circulating visfatin/NAMPT level. This indicates that circulating visfatin/NAMPT can be considered as a novel surrogate marker of systemic inflammation associated with fat depot, especially visceral, in the elderly population.
BACKGROUND: The available literature suggests that circulating visfatin/Nicotinamide Phosphoribosyltransferase (NAMPT) level variability in humans is related to obesity, insulin resistance, inflammation, and lipid profile. The aim of the study was to assess the relationship between circulating visfatin/NAMPT, obesity, insulin resistance, inflammation, and lipid profile in a large population-based, elderly cohort, applying structural equation modeling. MATERIALS AND METHODS: The analysis included 2983 elderly participants of the PolSenior study with assessed total blood count, fasting concentrations of lipids, glucose, insulin, hs-CRP, interleukin-6, and visfatin/NAMPT (by ELISA), and calculated HOMA-IR. RESULTS: The circulating visfatin/NAMPT levels were higher in obese compared to normal weight subjects, in those with hs-CRP above 3 mg/L, with low serum HDL cholesterol, and in insulin resistant subjects. Based on results of the exploratory factor analysis, a baseline model of mutual relationship between four latent and measured variables was created and a final model was developed by maintaining only two significant categories. The important variables for 'latent inflammation' proved to be hs-CRP and IL-6 serum levels. In the case of 'nutritional status', important variables were BMI, waist circumference, and to a lesser extent insulin resistance. Additionally, the residual correlation between those two constructs was also statistically significant. CONCLUSION: The structural equation modeling provided support for the existence of a link between nutritional status, inflammation and circulating visfatin/NAMPT level. This indicates that circulating visfatin/NAMPT can be considered as a novel surrogate marker of systemic inflammation associated with fat depot, especially visceral, in the elderly population.
Authors: Roldan M de Guia; Marianne Agerholm; Thomas S Nielsen; Leslie A Consitt; Ditte Søgaard; Jørn W Helge; Steen Larsen; Josef Brandauer; Joseph A Houmard; Jonas T Treebak Journal: Physiol Rep Date: 2019-07