Literature DB >> 27709567

Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.

Rongfang Xu1, Pengcheng Wei2, Jianbo Yang1.   

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) system is a newly emerging mutagenesis (gene-editing) tool in genetic engineering. Among the agriculturally important crops, several genes have been successfully mutated by the system, and some agronomic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this chapter, we describe a standard gene-editing procedure to effectively target rice genes and to make specific rice mutants using the CRISPR/Cas9 system mediated by Agrobacterium transformation.

Entities:  

Keywords:  Agrobacterium transformation; CRISPR/Cas9; Mutagenesis; Rice

Mesh：

Substances：

Year:  2017        PMID： 27709567     DOI： 10.1007/978-1-4939-6472-7_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  SALT AND ABA RESPONSE ERF1 improves seed germination and salt tolerance by repressing ABA signaling in rice.

Authors:  Yuxiang Li; Jiahao Zhou; Zhe Li; Jinzhu Qiao; Ruidang Quan; Juan Wang; Rongfeng Huang; Hua Qin
Journal:  Plant Physiol       Date:  2022-06-01       Impact factor: 8.005

2.  OsQHB Improves Salt Tolerance by Scavenging Reactive Oxygen Species in Rice.

Authors:  Jiahao Zhou; Jinzhu Qiao; Juan Wang; Ruidang Quan; Rongfeng Huang; Hua Qin
Journal:  Front Plant Sci       Date:  2022-05-04       Impact factor: 6.627

3.  The Ubiquitin-Binding Protein OsDSK2a Mediates Seedling Growth and Salt Responses by Regulating Gibberellin Metabolism in Rice.

Authors:  Juan Wang; Hua Qin; Shirong Zhou; Pengcheng Wei; Haiwen Zhang; Yun Zhou; Yuchen Miao; Rongfeng Huang
Journal:  Plant Cell       Date:  2019-12-11       Impact factor: 11.277

Review 4.  Functional Studies of Plant Latex as a Rich Source of Bioactive Compounds: Focus on Proteins and Alkaloids.

Authors:  Joanna Gracz-Bernaciak; Oliwia Mazur; Robert Nawrot
Journal:  Int J Mol Sci       Date:  2021-11-17       Impact factor: 5.923

5.  In-Frame and Frame-Shift Editing of the Ehd1 Gene to Develop Japonica Rice With Prolonged Basic Vegetative Growth Periods.

Authors:  Mingji Wu; Huaqing Liu; Yan Lin; Jianmin Chen; Yanping Fu; Jiami Luo; Zhujian Zhang; Kangjing Liang; Songbiao Chen; Feng Wang
Journal:  Front Plant Sci       Date:  2020-03-19       Impact factor: 5.753
  5 in total

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