Sabrina Alves Fernandes1, Silvia Bona2,3, Carlos Thadeu Schmidt Cerski4, Norma Possa Marroni2,3,5,6, Claudio Augusto Marroni1,3,7. 1. Programa de Pós-Graduação em Hepatologia, Universidade Federal de Ciências da Saúde de Porto Alegre, RS, Brasil. 2. Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, RS, Brasil. 3. Laboratório de Hepatologia e Gastroenterologia, Hospital de Clínicas de Porto Alegre, RS, Brasil. 4. Departamento de Patologia, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, RS, Brasil. 5. Programa de Pós-Graduação em Ciências Biológicas: Fisiologia, Universidade Federal do Rio Grande do Sul, RS, Brasil. 6. Programa de Pós-Graduação em Bio-Saúde, Universidade Luterana do Brasil, RS, Brasil. 7. Grupo adulto de Transplante de Fígado, Santa Casa de Porto Alegre, RS, Brasil.
Abstract
BACKGROUND: The inherent complications of cirrhosis include protein-calorie malnutrition and micronutrient deficiencies.Changes in taste are detrimental to the nutritional status, and the mechanism to explain these changes is not well documented in the cirrhotic patients. OBJECTIVE: To evaluate the taste buds of cirrhotic rats. METHODS: Fourteen male Wistar rats were evaluated. After 16 weeks, the liver was removed to histologically diagnose cirrhosis, and blood was collected to perform liver integrity tests. The tongue was removed for histological examination and immunohistochemistry using antibodies against protein gene product PGP 9.5 and the sweet taste receptors T1R2 and T1R3. Morphological changes were determined by scanning electron microscopy. Serum zinc levels were measured. RESULTS: The cirrhotic animals, but not the control animals, exhibited zinc deficiency. In both groups, there was positive immunoreactivity for type II and III cells and T1R2 receptors. The cirrhotic animals had no immunoreactivity for T1R3 receptors. Scanning electron microscopy analysis of the cirrhotic group revealed a uniform tapering of the gustatory papillae. CONCLUSION: In conclusion the experimental cirrhosis model mimicked the biochemical and histological parameters of human cirrhosis, therefore enabling a study of the gustatory papillae and taste buds.
BACKGROUND: The inherent complications of cirrhosis include protein-calorie malnutrition and micronutrient deficiencies.Changes in taste are detrimental to the nutritional status, and the mechanism to explain these changes is not well documented in the cirrhotic patients. OBJECTIVE: To evaluate the taste buds of cirrhotic rats. METHODS: Fourteen male Wistar rats were evaluated. After 16 weeks, the liver was removed to histologically diagnose cirrhosis, and blood was collected to perform liver integrity tests. The tongue was removed for histological examination and immunohistochemistry using antibodies against protein gene product PGP 9.5 and the sweet taste receptors T1R2 and T1R3. Morphological changes were determined by scanning electron microscopy. Serum zinc levels were measured. RESULTS: The cirrhotic animals, but not the control animals, exhibited zinc deficiency. In both groups, there was positive immunoreactivity for type II and III cells and T1R2 receptors. The cirrhotic animals had no immunoreactivity for T1R3 receptors. Scanning electron microscopy analysis of the cirrhotic group revealed a uniform tapering of the gustatory papillae. CONCLUSION: In conclusion the experimental cirrhosis model mimicked the biochemical and histological parameters of human cirrhosis, therefore enabling a study of the gustatory papillae and taste buds.
Authors: Silvia Bona; Sabrina Alves Fernandes; Andrea C Janz Moreira; Graziella Rodrigues; Elizângela G Schemitt; Fabio Cangeri Di Naso; Cláudio A Marroni; Norma P Marroni Journal: World J Gastrointest Pharmacol Ther Date: 2022-03-05
Authors: Fatuma Meyer; Karen Bannert; Mats Wiese; Susanne Esau; Lea F Sautter; Luise Ehlers; Ali A Aghdassi; Cornelia C Metges; Leif-A Garbe; Robert Jaster; Markus M Lerch; Georg Lamprecht; Luzia Valentini Journal: Int J Mol Sci Date: 2020-07-28 Impact factor: 5.923