Versatile perfusion chamber for high-magnification microscopy.

The large number of culture or perfusion chambers reflects the necessity of researchers to develop them from time to time because of specific biologic or optical requirements. The paper described a new versatile perfusion chamber, which can be machined at low cost and with a very rapid assembly procedure. An additional interesting feature is the lock system with an annular threaded screw that fits the angle of the objective, thus increasing the field of observation.