| Literature DB >> 27698243 |
Michael R Green, Joseph Sambrook.
Abstract
In this protocol, plasmid DNA is isolated from small-scale (1-2 mL) bacterial cultures. Yields vary between 100 and 5 µg of DNA, depending on the copy number of the plasmid. Miniprep DNA is sufficiently pure for use as a substrate or template in many in vitro enzymatic reactions. However, further purification is required if the plasmid DNA is used as the substrate in sequencing reactions.Entities:
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Year: 2016 PMID： 27698243 DOI： 10.1101/pdb.prot093344
Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095