| Literature DB >> 27696226 |
Maryam Pilehchian Langroudi1,2, Novin Nikbakhsh3, Ali Akbar Samadani2, Sadegh Fattahi2, Hassan Taheri4, Shahryar Shafaei5, Galia Amirbozorgi6, Reza Pilehchian Langroudi7, Haleh Akhavan-Niaki8,9.
Abstract
Gastric cancer is one of the major causes of death due to cancer in the world. It is a multi-factorial disease with epigenetic factors being also involved in its development. FAT4 is a tumor suppressor gene exerting an important role in cell adhesion. This study aimed at analyzing FAT4 expression and promoter methylation in gastric cancer. FAT4 expression was studied in 30 tumoral tissues and their non-tumoral counterparts using Taqman real time PCR method. Promoter methylation was assessed using bisulfite conversion method followed by sequencing. Tumor tissues showed reduced FAT4 expression (P = 0.04). FAT4 downregulation was associated with tumor grade, with higher repression at advanced grades. Significant increase of promoter methylation was observed in tumoral tissues. Reduced expression of FAT4 and increased methylation of its promoter may be one of the effective processes in turning a healthy stomach tissue into a tumor tissue.Entities:
Keywords: FAT4; Gastric cancer; Gene expression; Promoter methylation
Year: 2016 PMID： 27696226 PMCID： PMC5362569 DOI： 10.1007/s12079-016-0355-5
Source DB: PubMed Journal: J Cell Commun Signal ISSN： 1873-9601 Impact factor: 5.782