Literature DB >> 27692899

Isothermal Amplification Strategies for Detection in Microfluidic Devices.

Saheli Sarkar1, Pooja Sabhachandani1, Tania Konry2.   

Abstract

Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen-antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection. Copyright Â
© 2016 Elsevier Ltd. All rights reserved.

Keywords:  droplets; immuno-rolling circle amplification; microfluidics; protein

Mesh:

Substances:

Year:  2016        PMID: 27692899     DOI: 10.1016/j.tibtech.2016.09.006

Source DB:  PubMed          Journal:  Trends Biotechnol        ISSN: 0167-7799            Impact factor:   19.536


  3 in total

1.  Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions.

Authors:  Yuri Ishigaki; Kae Sato
Journal:  Micromachines (Basel)       Date:  2018-05-30       Impact factor: 2.891

Review 2.  Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics.

Authors:  Axel Hochstetter
Journal:  Micromachines (Basel)       Date:  2020-04-29       Impact factor: 2.891

3.  Rolling Circle Amplification as a Universal Method for the Analysis of a Wide Range of Biological Targets.

Authors:  R R Garafutdinov; A R Sakhabutdinova; A R Gilvanov; A V Chemeris
Journal:  Russ J Bioorg Chem       Date:  2021-12-16       Impact factor: 0.796

  3 in total

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