Elvira Román1, Daniel Prieto1, Ry Martin1, Inês Correia1, Ana Cecilia Mesa Arango2, Rebeca Alonso-Monge1, Oscar Zaragoza3, Jesús Pla1. 1. Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza de Ramón y Cajal s/n, E-28040 Madrid, Spain. 2. Group of Investigative Dermatology, University of Antioquía, Medellín, Colombia. 3. Mycology Reference Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
Abstract
AIM: To investigate the role of Cat1 overproduction in Candida albicans. MATERIALS & METHODS: Strains overproducing the CAT1 gene were constructed. RESULTS: Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen species generated by amphotericin B or ciclopirox olamine. CAT1 overproduction did not change the minimum inhibitory concentration of fungal cells to fungistatic or fungicidal azoles nor to amphotericin B although increased twofold the minimum inhibitory concentration to caspofungin. The role of Cat1 overproduction in virulence and colonization was also analyzed in mouse models. CONCLUSION: The overproduction of Cat1 protects against oxidants, phagocytes and certain antifungals at subinhibitory concentration but does not increase virulence in a systemic infection mouse model.
AIM: To investigate the role of Cat1 overproduction in Candida albicans. MATERIALS & METHODS: Strains overproducing the CAT1 gene were constructed. RESULTS: Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen species generated by amphotericin B or ciclopirox olamine. CAT1 overproduction did not change the minimum inhibitory concentration of fungal cells to fungistatic or fungicidal azoles nor to amphotericin B although increased twofold the minimum inhibitory concentration to caspofungin. The role of Cat1 overproduction in virulence and colonization was also analyzed in mouse models. CONCLUSION: The overproduction of Cat1 protects against oxidants, phagocytes and certain antifungals at subinhibitory concentration but does not increase virulence in a systemic infectionmouse model.
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Authors: Ana Morales-Menchén; Federico Navarro-García; José P Guirao-Abad; Elvira Román; Daniel Prieto; Ioana V Coman; Jesús Pla; Rebeca Alonso-Monge Journal: Front Cell Infect Microbiol Date: 2018-05-03 Impact factor: 5.293