Fabiano Luiz Heggendorn1, Lúcio de Souza Gonçalves2, Eliane Pedra Dias1, Christiane Heggendorn3, Márcia T S Lutterbach4. 1. Department of Pathology, Medical School, Fluminense Federal University, Rio de Janeiro, Brazil . 2. Dental School, Estácio de Sá University, Rio de Janeiro, Brazil . 3. Stomatologist, Fluminense Federal University, Rio de Janeiro, Brazil . 4. Laboratory of Biocorrosion and Biodegradation, National Institute of Technology.
Abstract
AIM: To detect for the presence of sulphate-reducing bacteria (SRB) and evaluate the possible association between SRB and cultivable facultative bacterial of oral sites with different periodontal conditions. METHODS: The study was carried out on 9 samples from different oral sites in 8 patients (two samples were collected from the same patient). Material was collected using modified Postgate E culture medium, indicated for the growth and isolation of SRB. In addition, a reducing solution for anaerobic bacteria was used as a transport solution for facultative bacteria and identified by polymerase chain reaction amplification (PCR) and sequencing of the 16S rRNA gene. RESULTS: SRB was found in 3 patient samples: the first in a root fragment, the second in a root fragment and a healthy tooth with vertical bone loss and a mobility degree of 3; and the third in a healthy tooth extracted for orthodontic treatment. In the final patient, the cultivable facultative species Lactobacillus casei was identified. Other facultative bacterial species were identified in patient 5 (Kurthia Gibsonii) and patient 7 (Pseudomonas aeruginosa). CONCLUSIONS: The detection of SRB in different dental tissues with distinct periodontal features demonstrated that new studies need to be developed in order to determine the true role of SRB in the oral microbiota. In addition, it was possible to verify the presence of Lactobacillus casei together with SRB in one sample.
AIM: To detect for the presence of sulphate-reducing bacteria (SRB) and evaluate the possible association between SRB and cultivable facultative bacterial of oral sites with different periodontal conditions. METHODS: The study was carried out on 9 samples from different oral sites in 8 patients (two samples were collected from the same patient). Material was collected using modified Postgate E culture medium, indicated for the growth and isolation of SRB. In addition, a reducing solution for anaerobic bacteria was used as a transport solution for facultative bacteria and identified by polymerase chain reaction amplification (PCR) and sequencing of the 16S rRNA gene. RESULTS:SRB was found in 3 patient samples: the first in a root fragment, the second in a root fragment and a healthy tooth with vertical bone loss and a mobility degree of 3; and the third in a healthy tooth extracted for orthodontic treatment. In the final patient, the cultivable facultative species Lactobacillus casei was identified. Other facultative bacterial species were identified in patient 5 (Kurthia Gibsonii) and patient 7 (Pseudomonas aeruginosa). CONCLUSIONS: The detection of SRB in different dental tissues with distinct periodontal features demonstrated that new studies need to be developed in order to determine the true role of SRB in the oral microbiota. In addition, it was possible to verify the presence of Lactobacillus casei together with SRB in one sample.
Authors: Alisha G Campbell; James H Campbell; Patrick Schwientek; Tanja Woyke; Alexander Sczyrba; Steve Allman; Clifford J Beall; Ann Griffen; Eugene Leys; Mircea Podar Journal: PLoS One Date: 2013-03-26 Impact factor: 3.240
Authors: Lorelei Bozo-Hurtado; M Alexandra García-Amado; Andrei Chistoserdov; Ramon Varela; J Jesus Narvaez; Rita Colwell; Paula Suárez Journal: Aquat Biosyst Date: 2013-08-28