Omid Dianat1, Fatemeh Mashhadi Abas2, Payam Paymanpour3, Mohammad Jafar Eghbal4, Sahar Haddadpour5, Nazanin Bahrololumi6. 1. Iranian Center for Endodontic Research, Research Institute of Dental Science, Endodontic Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Pathology Department, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Endodontic Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4. Iranian Center for Endodontic Research, Research Institute of Dental Sciences Endodontic Department, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 5. Orthodontic Department, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 6. Research Institute of Dental Sciences, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND/AIM: Different types of scaffolds have been suggested for endodontic repair procedures. The aim of this study was to compare the outcome of procedures using blood clot vs plasma rich in growth factors (PRGF) scaffold in immature teeth with apical periodontitis in dogs. MATERIALS AND METHODS: In this in vivo animal study, 20 teeth (30 canals) with open apices were divided into two groups (n = 12): (1) blood clot and (2) PRGF. Two positive and negative control groups were also selected randomly. After exposing the pulp and inducing periapical inflammation by placing a sponge soaked in a suspension of dental plaque, the root canals of both groups were flushed with sodium hypochlorite and filled with triple antibiotic paste. After 28 days, the antibiotic mixture was removed, bleeding was induced through the apical foramen, and mineral trioxide aggregate was placed over the blood clot in group one and PRGF scaffold in group two. Access cavities were then filled with composite resin. The radiographic and histological findings were compared immediately after the procedure, as well as after 3 and 6 months of follow-up. RESULTS: There was no statistically significant difference between the two experimental groups with regard to the radiographic or histological results (P > 0.05). The incidence of healed periapical radiolucency was 60% and 53.33% in groups one and two, respectively. Apical closure was noted in 60% of samples in both experimental groups. New cementum-like tissue formation was detected along the walls of the canals, and bone-like islands within the canals were also observed. CONCLUSIONS: Plasma rich in growth factors scaffold showed no advantage over blood clots regarding healing of the periapical radiolucency, radiographic apical closure and type of the newly formed soft and hard tissues.
BACKGROUND/AIM: Different types of scaffolds have been suggested for endodontic repair procedures. The aim of this study was to compare the outcome of procedures using blood clot vs plasma rich in growth factors (PRGF) scaffold in immature teeth with apical periodontitis in dogs. MATERIALS AND METHODS: In this in vivo animal study, 20 teeth (30 canals) with open apices were divided into two groups (n = 12): (1) blood clot and (2) PRGF. Two positive and negative control groups were also selected randomly. After exposing the pulp and inducing periapical inflammation by placing a sponge soaked in a suspension of dental plaque, the root canals of both groups were flushed with sodium hypochlorite and filled with triple antibiotic paste. After 28 days, the antibiotic mixture was removed, bleeding was induced through the apical foramen, and mineral trioxide aggregate was placed over the blood clot in group one and PRGF scaffold in group two. Access cavities were then filled with composite resin. The radiographic and histological findings were compared immediately after the procedure, as well as after 3 and 6 months of follow-up. RESULTS: There was no statistically significant difference between the two experimental groups with regard to the radiographic or histological results (P > 0.05). The incidence of healed periapical radiolucency was 60% and 53.33% in groups one and two, respectively. Apical closure was noted in 60% of samples in both experimental groups. New cementum-like tissue formation was detected along the walls of the canals, and bone-like islands within the canals were also observed. CONCLUSIONS: Plasma rich in growth factors scaffold showed no advantage over blood clots regarding healing of the periapical radiolucency, radiographic apical closure and type of the newly formed soft and hard tissues.