| Literature DB >> 276868 |
J L Campbell, C C Richardson, F W Studier.
Abstract
Frafments of phage T7 DNA have been cloned in Escherichia coli by using the plasmid pMB9. Such cloned fragments are able to recombine with infecting phages, thus providing a means to integrate the physical and genetic maps of T7 DNA. Approximately 65% of the T7 DNA molecule has been found in clones so far, and analysis of these clones has mapped genes 12-17 with an accuracy of about 1% the total length of T7 DNA. At least some cloned segments can supply T7 functions to infecting phages.Entities:
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Year: 1978 PMID: 276868 PMCID: PMC392535 DOI: 10.1073/pnas.75.5.2276
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205