Najla E Farage1, Milena B Stockler-Pinto2, Viviane O Leal3, Ludmila Lmf Cardozo2, José Carlos Carraro-Eduardo4, Denis Fouque5, Denise Mafra1,2. 1. Medical Sciences Graduate Program, Federal Fluminense University (UFF), Niterói, RJ, Brazil. 2. Programa de Pós-graduação em Ciências Cardiovasculares, Hospital Universitário Antônio Pedro, Universidade Federal Fluminense, Rua Marques do Paraná, 303, 4o andar, Niterói, RJ, ZC: 24033-900, Brazil. 3. Programa de Pós-graduação em Ciências Cardiovasculares, Hospital Universitário Antônio Pedro, Universidade Federal Fluminense, Rua Marques do Paraná, 303, 4o andar, Niterói, RJ, ZC: 24033-900, Brazil. vivianeoleal@yahoo.com.br. 4. Medicine Faculty, UFF, Niterói, RJ, Brazil. 5. Department of Nephrology, Centre Hopitalier Lyon Sud, Pierre-Bénite, France.
Abstract
PURPOSE: This study aimed to evaluate the association among the expressions of pro- and anti-inflammatory nuclear factors (nuclear factor-kappaB, NF-κB and nuclear erythroid 2-related factor 2, Nrf2) and nutritional status in HD patients. METHODS: This cross-sectional study included eighty-three HD patients. The peripheral blood mononuclear cells were isolated and processed for the evaluation of NF-κB and Nrf2 RNAm expression by quantitative real-time polymerase chain reaction. Muscle mass was estimated by creatinine index (CI) and percentage of body fat (%BF) by anthropometry. Seven-point subjective global assessment was also used to evaluate the nutritional status. RESULTS: The NF-κB expression was negatively correlated with CI (r = -0.54, p = 0.0001), serum albumin (r = -0.32, p = 0.02) and %BF (r = -0.61, p = 0.001). Multiple linear regression analysis revealed that NF-κB expression was independently associated with CI (β: -0.8, p = 0.013) and %BF (β: -0.42, p = 0.04). There was no correlation among Nrf2 and anthropometric and biochemical variables. CONCLUSION: The classical NF-κB activation seems to be associated with poor nutritional status in HD patients; however, the exact underlying mechanisms deserve further studies.
PURPOSE: This study aimed to evaluate the association among the expressions of pro- and anti-inflammatory nuclear factors (nuclear factor-kappaB, NF-κB and nuclear erythroid 2-related factor 2, Nrf2) and nutritional status in HDpatients. METHODS: This cross-sectional study included eighty-three HDpatients. The peripheral blood mononuclear cells were isolated and processed for the evaluation of NF-κB and Nrf2 RNAm expression by quantitative real-time polymerase chain reaction. Muscle mass was estimated by creatinine index (CI) and percentage of body fat (%BF) by anthropometry. Seven-point subjective global assessment was also used to evaluate the nutritional status. RESULTS: The NF-κB expression was negatively correlated with CI (r = -0.54, p = 0.0001), serum albumin (r = -0.32, p = 0.02) and %BF (r = -0.61, p = 0.001). Multiple linear regression analysis revealed that NF-κB expression was independently associated with CI (β: -0.8, p = 0.013) and %BF (β: -0.42, p = 0.04). There was no correlation among Nrf2 and anthropometric and biochemical variables. CONCLUSION: The classical NF-κB activation seems to be associated with poor nutritional status in HDpatients; however, the exact underlying mechanisms deserve further studies.
Authors: Liliana M Pedruzzi; Ludmila F M F Cardozo; Julio B Daleprane; Milena B Stockler-Pinto; Elisa B Monteiro; Maurilo Leite; Nosratola D Vaziri; Denise Mafra Journal: J Nephrol Date: 2015-01-14 Impact factor: 3.902