Literature DB >> 27671941

Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR).

Sunil Chandran1, Elaine Shapland2.   

Abstract

The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction.

Entities:  

Keywords:  DNA assembly; DNA synthesis; High throughput; Synthetic biology; Yeast homologous recombination

Mesh：

Year:  2017        PMID： 27671941     DOI： 10.1007/978-1-4939-6343-0_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  An exclusive metabolic niche enables strain engraftment in the gut microbiota.

Authors:  Elizabeth Stanley Shepherd; William C DeLoache; Kali M Pruss; Weston R Whitaker; Justin L Sonnenburg
Journal:  Nature       Date:  2018-05-09       Impact factor: 49.962

2.  Genome Partitioner: A web tool for multi-level partitioning of large-scale DNA constructs for synthetic biology applications.

Authors:  Matthias Christen; Luca Del Medico; Heinz Christen; Beat Christen
Journal:  PLoS One       Date:  2017-05-22       Impact factor: 3.240

3.  An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals.

Authors:  Pablo Carbonell; Adrian J Jervis; Christopher J Robinson; Cunyu Yan; Mark Dunstan; Neil Swainston; Maria Vinaixa; Katherine A Hollywood; Andrew Currin; Nicholas J W Rattray; Sandra Taylor; Reynard Spiess; Rehana Sung; Alan R Williams; Donal Fellows; Natalie J Stanford; Paul Mulherin; Rosalind Le Feuvre; Perdita Barran; Royston Goodacre; Nicholas J Turner; Carole Goble; George Guoqiang Chen; Douglas B Kell; Jason Micklefield; Rainer Breitling; Eriko Takano; Jean-Loup Faulon; Nigel S Scrutton
Journal:  Commun Biol       Date:  2018-06-08

4.  SEVA 3.1: enabling interoperability of DNA assembly among the SEVA, BioBricks and Type IIS restriction enzyme standards.

Authors:  Stamatios G Damalas; Christos Batianis; Maria Martin-Pascual; Victor de Lorenzo; Vitor A P Martins Dos Santos
Journal:  Microb Biotechnol       Date:  2020-07-25       Impact factor: 5.813

Review 5.  Designing with living systems in the synthetic yeast project.

Authors:  Erika Szymanski; Jane Calvert
Journal:  Nat Commun       Date:  2018-07-27       Impact factor: 14.919
  5 in total

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