| Literature DB >> 27671938 |
Abstract
Clonetegration is a method for site-specific insertion of DNA into prokaryotic chromosomes, based on bacteriophage integrases. The method combines DNA cloning/assembly and chromosomal integration into a single step, providing a simple and rapid strategy for inserting DNA sequences into bacterial chromosomes.Entities:
Keywords: Bacteriophage integrase; Clonetegration; Genome editing; Genome engineering; Synthetic biology
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Year: 2017 PMID： 27671938 DOI： 10.1007/978-1-4939-6343-0_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745