Jung-Hwan Lee1, Ahmed El-Fiqi2, Jeong-Ki Jo3, Dong-Ae Kim3, Si-Chul Kim3, Soo-Kyung Jun4, Hae-Won Kim5, Hae-Hyoung Lee6. 1. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea. 2. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Research Center, Dankook University, Cheonan 330-714, South Korea. 3. Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea. 4. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea. 5. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine Research Center, Dankook University, Cheonan 330-714, South Korea; Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea. 6. Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea; Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea. Electronic address: haelee@dku.edu.
Abstract
OBJECTIVE: Poly(methyl methacrylate) (PMMA) used as removable denture bases or orthodontic appliances has relatively poor antimicrobial properties, which accelerate oral infection and induce unfavorable odors. Mesoporous silica nanoparticles (MSNs) have been highlighted as a potential additive to overcome this issue because of their drug-loading capacity. Here, we present the long-term antimicrobial effect of MSN-incorporated PMMA with drug-loading capacity. METHODS: After the MSNs were characterized, MSN incorporation into chemically activated PMMA (0.5, 1, 2.5 or 5wt%) relative to the methyl methacrylate powder by mass was fabricated into a rectangular specimen (1.4×3.0×19.0mm) for a 3-point flexural test at a speed of 1mm/min or a disk (∅=11.5mm and d=1.5mm) for investigation of its antimicrobial effects. RESULTS: A typical spherical morphology with a well-ordered mesoporous structure of the MSNs was visualized and is beneficial for loading drugs and combining in matrixes. Among the tested levels of MSN incorporation in PMMA (0.5, 1, 2.5 or 5wt%), only 5wt% decreased the flexural strength (p<0.05), whereas the flexural modulus was not significantly decreased (p>0.05). The surface roughness and surface energy were increased with 2.5wt% or 5wt% incorporation. An anti-adherent effect against Candida albicans and Streptococcus oralis after 1h of attachment was only observed with 2.5 and 5wt% incorporation compared to a lack of MSNs (p<0.05). A long-term antimicrobial effect was observed for 2 weeks with 2.5wt% MSN-incorporated PMMA when amphotericin B was loaded into the MSNs on the PMMA surface. SIGNIFICANCE: The long-term antimicrobial performance after loading amphotericin B into the MSN-incorporated PMMA suggests the potential clinical usefulness of MSN-incorporated PMMA resin. Copyright Â
OBJECTIVE:Poly(methyl methacrylate) (PMMA) used as removable denture bases or orthodontic appliances has relatively poor antimicrobial properties, which accelerate oral infection and induce unfavorable odors. Mesoporous silica nanoparticles (MSNs) have been highlighted as a potential additive to overcome this issue because of their drug-loading capacity. Here, we present the long-term antimicrobial effect of MSN-incorporated PMMA with drug-loading capacity. METHODS: After the MSNs were characterized, MSN incorporation into chemically activated PMMA (0.5, 1, 2.5 or 5wt%) relative to the methyl methacrylate powder by mass was fabricated into a rectangular specimen (1.4×3.0×19.0mm) for a 3-point flexural test at a speed of 1mm/min or a disk (∅=11.5mm and d=1.5mm) for investigation of its antimicrobial effects. RESULTS: A typical spherical morphology with a well-ordered mesoporous structure of the MSNs was visualized and is beneficial for loading drugs and combining in matrixes. Among the tested levels of MSN incorporation in PMMA (0.5, 1, 2.5 or 5wt%), only 5wt% decreased the flexural strength (p<0.05), whereas the flexural modulus was not significantly decreased (p>0.05). The surface roughness and surface energy were increased with 2.5wt% or 5wt% incorporation. An anti-adherent effect against Candida albicans and Streptococcus oralis after 1h of attachment was only observed with 2.5 and 5wt% incorporation compared to a lack of MSNs (p<0.05). A long-term antimicrobial effect was observed for 2 weeks with 2.5wt% MSN-incorporated PMMA when amphotericin B was loaded into the MSNs on the PMMA surface. SIGNIFICANCE: The long-term antimicrobial performance after loading amphotericin B into the MSN-incorporated PMMA suggests the potential clinical usefulness of MSN-incorporated PMMA resin. Copyright Â
Authors: Hang-Nga Mai; Do-Yeon Kim; Dong Choon Hyun; Ju Hayng Park; Sang Min Lee; Du-Hyeong Lee Journal: J Clin Med Date: 2019-11-01 Impact factor: 4.241