Rishi Pal1, Manju J Chaudhary2, Prafulla Chandra Tiwari3, Rajendra Nath3, Suresh Babu4, Kamlesh Kumar Pant3. 1. Department of Pharmacology & Therapeutics, King George's Medical University, Lucknow, UP, 226003, India. rishipal@kgmcindia.edu. 2. Department of Physiology, Government Medical College, Tirwa Road, Kannauj, UP, India. 3. Department of Pharmacology & Therapeutics, King George's Medical University, Lucknow, UP, 226003, India. 4. Department of Pathology, King George's Medical University, Lucknow, UP, 226003, India.
Abstract
AIM: Present study was designed to evaluate protective effects of pentoxifylline and its potentiation with low dose of nitric oxide (NO) modulators in adjuvant-induced experimental arthritis in rats. METHOD: Wistar rats (200-300 g, n = 8 per group) of both sexes were used in the study. On day "0" experimental arthritis was induced by injecting 0.2 ml of Complete Freund's adjuvant (CFA) in sub-planter region of right hind paw of animals. Pentoxifylline treatment alone and in combination with NO modulators was given (i.p.) from day 14 to 28. Various arthritic parameters were recorded and blood and joint synovial fluid was collected for biochemical analysis. RESULTS: CFA inoculation significantly increases (1) arthritic index (2) ankle diameter (3) paw volume (4) histopathology score (5) serum TNF-α, IL-6, IL-1β and synovial TNF-α levels (p < 0.001) (6) serum Th1 and Th2 cytokine levels g) MDA levels in rat paw tissue homogenates (7) serum NF-κB levels. Significant decrease in serum IL-10 levels and SOD activity was observed in rats after CFA inoculation. Decrease in body weight and suppressed general quality of life of CFA inoculated rats was also observed. These CFA-induced arthritic changes were significantly reversed by pentoxifylline alone and in combination with low dose of NO modulators (p < 0.05). CONCLUSION: These results are suggestive of protective effects of pentoxifylline and its potentiation in combination with low dose of NO modulators. These results may provide new pharmacological therapy for management of rheumatoid arthritis (RA).
AIM: Present study was designed to evaluate protective effects of pentoxifylline and its potentiation with low dose of nitric oxide (NO) modulators in adjuvant-induced experimental arthritis in rats. METHOD:Wistar rats (200-300 g, n = 8 per group) of both sexes were used in the study. On day "0" experimental arthritis was induced by injecting 0.2 ml of Complete Freund's adjuvant (CFA) in sub-planter region of right hind paw of animals. Pentoxifylline treatment alone and in combination with NO modulators was given (i.p.) from day 14 to 28. Various arthritic parameters were recorded and blood and joint synovial fluid was collected for biochemical analysis. RESULTS: CFA inoculation significantly increases (1) arthritic index (2) ankle diameter (3) paw volume (4) histopathology score (5) serum TNF-α, IL-6, IL-1β and synovial TNF-α levels (p < 0.001) (6) serum Th1 and Th2 cytokine levels g) MDA levels in rat paw tissue homogenates (7) serum NF-κB levels. Significant decrease in serum IL-10 levels and SOD activity was observed in rats after CFA inoculation. Decrease in body weight and suppressed general quality of life of CFA inoculated rats was also observed. These CFA-induced arthritic changes were significantly reversed by pentoxifylline alone and in combination with low dose of NO modulators (p < 0.05). CONCLUSION: These results are suggestive of protective effects of pentoxifylline and its potentiation in combination with low dose of NO modulators. These results may provide new pharmacological therapy for management of rheumatoid arthritis (RA).
Authors: T Luna; S B Santos; M Nascimento; M A F Porto; A L Muniz; E M Carvalho; A R Jesus Journal: Braz J Med Biol Res Date: 2011-10-22 Impact factor: 2.590
Authors: S Stosić-Grujicić; D Maksimović; V Badovinac; T Samardzić; V Trajković; M Lukić; M Mostarica Stojković Journal: J Autoimmun Date: 2001-02 Impact factor: 7.094
Authors: Celso M Queiroz-Junior; Rafaela L C Bessoni; Vivian V Costa; Danielle G Souza; Mauro M Teixeira; Tarcília A Silva Journal: Life Sci Date: 2013-08-02 Impact factor: 5.037