| Literature DB >> 27670110 |
Yoshifumi Wakisaka1, Yuta Suzuki2, Osamu Iwata3, Ayaka Nakashima3, Takuro Ito4,5, Misa Hirose6, Ryota Domon6, Mai Sugawara6, Norimichi Tsumura6, Hiroshi Watarai7, Tomoyoshi Shimobaba8, Kengo Suzuki3, Keisuke Goda1,9,10, Yasuyuki Ozeki2.
Abstract
Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.Entities:
Mesh：
Substances：
Year: 2016 PMID： 27670110 DOI： 10.1038/nmicrobiol.2016.124
Source DB: PubMed Journal: Nat Microbiol ISSN： 2058-5276 Impact factor: 17.745