Attia Anwar1, Marina Marini2,3, Provvidenza Maria Abruzzo2,3, Alessandra Bolotta2,3, Alessandro Ghezzo2, Paola Visconti4, Paul J Thornalley1,5, Naila Rabbani5. 1. a Warwick Medical School, Clinical Sciences Research Laboratories , University of Warwick, University Hospital Coventry , Coventry , UK. 2. b Department of Experimental, Diagnostic and Specialty Medicine , School of Medicine, University of Bologna , Bologna , Italy. 3. c Don Carlo Gnocchi Foundation ONLUS, IRCCS "S. Maria Nascente" , Milan , Italy. 4. d Child Neurology and Psychiatry Unit , IRCCS Institute of Neurological Sciences , Bologna , Italy. 5. e Warwick Systems Biology, Clinical Sciences Research Laboratories , University of Warwick, University Hospital Coventry , Coventry , UK.
Abstract
AIMS/HYPOTHESIS: To assess thiamine and related metabolite status by analysis of plasma and urine in autistic children and healthy controls, correlations to clinical characteristics and link to plasma protein markers of oxidative damage. METHODS: 27 children with autism (21 males and 6 females) and 21 (15 males and 6 females) age-matched healthy control children were recruited. The concentration of thiamine and related phosphorylated metabolites in plasma and urine and plasma protein content of dityrosine, N-formylkynurenine and 3-nitrotyrosine was determined. RESULTS: Plasma thiamine and thiamine monophosphate concentrations were similar in both study groups (median [lower-upper quartile]): autistic children - 6.60 nM (4.48-8.91) and 7.00 nM (5.51-8.55), and healthy controls - 6.82 nM (4.47-7.02) and 6.82 nM (5.84-8.91), respectively. Thiamine pyrophosphate (TPP) was decreased 24% in autistic children compared to healthy controls: 6.82 nM (5.81-8.52) versus 9.00 nM (8.41-10.71), p < .01. Urinary excretion of thiamine and fractional renal clearance of thiamine did not change between the groups. No correlation was observed between clinical markers and the plasma and urine thiamine concentration. Plasma protein dityrosine content was increased 88% in ASD. Other oxidative markers were unchanged. CONCLUSIONS/ INTERPRETATION: Autistic children had normal plasma and urinary thiamine levels whereas plasma TPP concentration was decreased. The latter may be linked to abnormal tissue handling and/or absorption from gut microbiota of TPP which warrants further investigation. Increased plasma protein dityrosine may reflect increased dual oxidase activity in response to change in mucosal immunity and host-microbe homeostasis.
AIMS/HYPOTHESIS: To assess thiamine and related metabolite status by analysis of plasma and urine in autisticchildren and healthy controls, correlations to clinical characteristics and link to plasma protein markers of oxidative damage. METHODS: 27 children with autism (21 males and 6 females) and 21 (15 males and 6 females) age-matched healthy control children were recruited. The concentration of thiamine and related phosphorylated metabolites in plasma and urine and plasma protein content of dityrosine, N-formylkynurenine and 3-nitrotyrosine was determined. RESULTS: Plasma thiamine and thiamine monophosphate concentrations were similar in both study groups (median [lower-upper quartile]): autisticchildren - 6.60 nM (4.48-8.91) and 7.00 nM (5.51-8.55), and healthy controls - 6.82 nM (4.47-7.02) and 6.82 nM (5.84-8.91), respectively. Thiamine pyrophosphate (TPP) was decreased 24% in autisticchildren compared to healthy controls: 6.82 nM (5.81-8.52) versus 9.00 nM (8.41-10.71), p < .01. Urinary excretion of thiamine and fractional renal clearance of thiamine did not change between the groups. No correlation was observed between clinical markers and the plasma and urine thiamine concentration. Plasma protein dityrosine content was increased 88% in ASD. Other oxidative markers were unchanged. CONCLUSIONS/ INTERPRETATION:Autisticchildren had normal plasma and urinary thiamine levels whereas plasma TPP concentration was decreased. The latter may be linked to abnormal tissue handling and/or absorption from gut microbiota of TPP which warrants further investigation. Increased plasma protein dityrosine may reflect increased dual oxidase activity in response to change in mucosal immunity and host-microbe homeostasis.
Authors: Antonietta Messina; Vincenzo Monda; Francesco Sessa; Anna Valenzano; Monica Salerno; Ilaria Bitetti; Francesco Precenzano; Rosa Marotta; Francesco Lavano; Serena M Lavano; Margherita Salerno; Agata Maltese; Michele Roccella; Lucia Parisi; Roberta I Ferrentino; Gabriele Tripi; Beatrice Gallai; Giuseppe Cibelli; Marcellino Monda; Giovanni Messina; Marco Carotenuto Journal: Front Physiol Date: 2018-03-22 Impact factor: 4.566
Authors: Nagwa A Meguid; Said A S Ghozlan; Magda F Mohamed; Marwa K Ibrahim; Reham M Dawood; Noha G Bader El Din; Tawfeek H Abdelhafez; Maha Hemimi; Mostafa K El Awady Journal: Biomark Insights Date: 2017-02-23
Authors: P M Abruzzo; A Matté; A Bolotta; E Federti; A Ghezzo; T Guarnieri; M Marini; A Posar; A Siciliano; L De Franceschi; P Visconti Journal: J Transl Med Date: 2019-10-02 Impact factor: 5.531