Vanja Cejvanovic1,2, Camilla Asferg3,4, Laura Kofoed Kjær1, Ulrik B Andersen4, Allan Linneberg2,5,6, Jan Frystyk7,8, Trine Henriksen1, Allan Flyvbjerg7,8, Michael Christiansen9,10, Allan Weimann1, Jørgen Jeppesen2,3, Henrik Enghusen Poulsen1,2. 1. a Department of Clinical Pharmacology , Bispebjerg and Frederiksberg Hospital , Copenhagen , Denmark. 2. b Faculty of Health and Medical Sciences , University of Copenhagen , Copenhagen , Denmark. 3. c Department of Medicine , Hvidovre Hospital Glostrup , Copenhagen , Denmark. 4. d Department of Clinical Physiology, Nuclear Medicine and PET, Glostrup section , Rigshospitalet , Copenhagen , Denmark. 5. e Research Centre for Prevention and Health , the Capital Region of Denmark, Copenhagen , Denmark. 6. f Department of Clinical Experimental Research , Rigshospitalet and Glostrup Hospital , Copenhagen , Denmark. 7. g Medical Research Laboratory, Department of Clinical Medicine, Faculty of Health , Aarhus University , Aarhus , Denmark. 8. h Department of Endocrinology and Internal Medicine , Aarhus University Hospital , Aarhus , Denmark. 9. i Department for Congenital Disorders , Statens Serum Institut , Copenhagen , Denmark. 10. j Department of Biomedical Sciences , University of Copenhagen , Copenhagen , Denmark.
Abstract
OBJECTIVES: The aim of our study was to investigate if the 24-hour excretion of the urinary markers for oxidative stress to DNA and RNA, measured as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo), respectively, were increased in obese individuals with or without hypertension compared to lean controls. METHODS: A total of 63 obese hypertensive men (obeseHT), 40 obese normotensive men (obeseNT) and 27 lean normotensive men (leanNT) were included in the study. Body mass index (BMI) was between 20.0 and 24.9 kg/m2 in leanNT participants and ≥30 kg/m2 in obese participants. Hypertension was defined as a mean 24-hour systolic ambulatory blood pressure (AMBP) ≥ 130 mmHg or a mean 24-hour diastolic AMBP ≥80 mmHg and normotension as mean 24-hour AMBP <130/80 mmHg. Twenty-four hour urinary 8-oxoGuo and 8-oxodG excretion (nmol/24 h) were measured by a validated liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS). RESULTS: Urinary 8-oxoGuo excretion was (median and [interquartile range]) 30.8 [27.8-32.2] nmol/24 h in leanNT, 36.8 [31.3-40.2] nmol/24 h in obeseNT and 40.6 [31.7-48.5] nmol/24 h in obeseHT. The difference was statistically significant (p = .002) and post hoc tests showed a significant difference between leanNT and obeseHT (p = .001) as well as obeseNT (p = .002), whereas the two obese groups did not differ (p = .6). No statistically significant differences in 8-oxodG concentrations were observed between the three groups (p = .3). CONCLUSION: The measurement of urinary excretion of 8-oxoGuo suggests that obesity in men, but not hypertension, is associated with increased oxidative damage to RNA.
OBJECTIVES: The aim of our study was to investigate if the 24-hour excretion of the urinary markers for oxidative stress to DNA and RNA, measured as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo), respectively, were increased in obese individuals with or without hypertension compared to lean controls. METHODS: A total of 63 obese hypertensivemen (obeseHT), 40 obese normotensive men (obeseNT) and 27 lean normotensive men (leanNT) were included in the study. Body mass index (BMI) was between 20.0 and 24.9 kg/m2 in leanNT participants and ≥30 kg/m2 in obeseparticipants. Hypertension was defined as a mean 24-hour systolic ambulatory blood pressure (AMBP) ≥ 130 mmHg or a mean 24-hour diastolic AMBP ≥80 mmHg and normotension as mean 24-hour AMBP <130/80 mmHg. Twenty-four hour urinary 8-oxoGuo and 8-oxodG excretion (nmol/24 h) were measured by a validated liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS). RESULTS: Urinary 8-oxoGuo excretion was (median and [interquartile range]) 30.8 [27.8-32.2] nmol/24 h in leanNT, 36.8 [31.3-40.2] nmol/24 h in obeseNT and 40.6 [31.7-48.5] nmol/24 h in obeseHT. The difference was statistically significant (p = .002) and post hoc tests showed a significant difference between leanNT and obeseHT (p = .001) as well as obeseNT (p = .002), whereas the two obese groups did not differ (p = .6). No statistically significant differences in 8-oxodG concentrations were observed between the three groups (p = .3). CONCLUSION: The measurement of urinary excretion of 8-oxoGuo suggests that obesity in men, but not hypertension, is associated with increased oxidative damage to RNA.
Authors: Margalida Monserrat-Mesquida; Magdalena Quetglas-Llabrés; Cristina Bouzas; Silvia García; David Mateos; Cristina Gómez; José M Gámez; Henrik E Poulsen; Josep A Tur; Antoni Sureda Journal: Antioxidants (Basel) Date: 2022-07-05